Copy Link Print Information

Myelin Basic Protein Antibody (YA5706)

Customer Review

Based on 1 Customer Validation

Myelin Basic Protein Antibody (YA5706) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myelin Basic Protein.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Myelin Basic Protein Antibody (YA5706) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-400 1:500-5000

Product Details

Description

Myelin Basic Protein Antibody (YA5706) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myelin Basic Protein.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Synthesized peptide derived from human Myelin Basic Protein(MBP) AA range: 150-250

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Myelin Basic Protein is a The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation

  • Subcellular Localization

    Myelin membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus

  • Expression


    Tissue_specificity:MBP isoforms are found in the central and peripheral nervous systems, while the Golli-MBP isoform is expressed in the fetal thymus, spleen, spinal cord, and cell lines derived from the immune system.

  • Isoforms & Post-Translational Modification

    P02686 has 6 isomers: P02686-1: 33117 Da (predicted); P02686-2: 21522 Da (predicted); P02686-3: 21493 Da (predicted); P02686-4: 20246 Da (predicted); P02686-5: 18591 Da (predicted); P02686-6: 17343 Da (predicted).
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic;The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6);Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated;Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells;Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1

  • Subunit

    Homodimer. Isoform 3 exists as a homodimer

  • SwissProt ID

    P02686

  • Gene ID
    4155 [NCBI]

Myelin Basic Protein Antibody (YA5706) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    1/16
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Myelin Basic Protein Antibody (YA5706) Product Image
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Experimental Validation Results for Myelin Basic Protein Antibody (YA5706)
  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Myelin Basic Protein Antibody (HY-P86014, 1/400). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P
  • Myelin Basic Protein Antibody (YA5706) Product Image IHC-P