p27 KIP 1 Antibody (YA5002)(PBS only)

Cat. No.: HY-P85310A: Fiche technique User Guide for Antibodies
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p27 KIP 1 Antibody (YA5002) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to p27 KIP 1.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

* Veuillez sélectionner la quantité avant d'ajouter des articles.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

p27 KIP 1 Antibody (YA5002) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to p27 KIP 1.

Host

Mouse

Masse moléculaire

Predicted band size: 22 kDa;

Species Reactivity

Human

SwissProt ID

P46527

Gene ID

1027 [NCBI]

Immunogen

Recombinant human CDKN1B. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Pureté	affinity purified by Protein A	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry

Subcellular Localization：Nucleus; Cytoplasm; Endosome

Expression：
Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .

Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase

Subunit：Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.

Synonyms

CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Documentation

User Guide for Antibodies (1077 KB)