PRAS40 Antibody (YA4417)(PBS only)

Cat. No.: HY-P84720A: Data Sheet User Guide for Antibodies
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PRAS40 Antibody (YA4417) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRAS40.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PRAS40 Antibody (YA4417) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRAS40.

Host

Mouse

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96B36

Gene ID

84335 [NCBI]

Immunogen

Purified recombinant fragment of human AKT1S1 (AA: 92-276) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:17277771, PubMed:17386266, PubMed:17510057, PubMed:29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed:29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed:17386266, PubMed:18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed:16174443, PubMed:18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:It is widely expressed, with the highest expression levels in the liver and heart. Expression levels in cancer cell lines (e.g., A-549 and HeLa) are higher than in normal cell lines (e.g., HEK293) .

Isoforms & Post-Translational Modification：Q96B36 has 3 isomers: Q96B36-1: 27383 Da (predicted); Q96B36-2: 13807 Da (predicted); Q96B36-3: 29262 Da (predicted).
Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity (PubMed:12524439, PubMed:17386266). Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation (PubMed:17517883). Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227)

Subunit：Associated component of the mechanistic target of rapamycin complex 1 (mTORC1), which contains core MTOR, MLST8 and RPTOR (PubMed:17277771, PubMed:17386266, PubMed:17510057, PubMed:29236692, PubMed:31601764). Dissociates from mTORC1 in response to insulin treatment (PubMed:17386266, PubMed:18372248). mTORC1 binds to and is inhibited by FKBP12-rapamycin (PubMed:17277771, PubMed:31601764). Interacts (via TOS motif) with RPTOR; interaction is direct (PubMed:17386266, PubMed:17510057, PubMed:17517883). The phosphorylated form interacts with 14-3-3 proteins (PubMed:12524439, PubMed:18372248)

Synonyms

Lobe; PRAS40

Documentation

User Guide for Antibodies (1077 KB)