Rtn-3 Antibody (YA5050)(PBS only)

Cat. No.: HY-P85358A: Data Sheet User Guide for Antibodies
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Rtn-3 Antibody (YA5050) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Rtn-3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Rtn-3 Antibody (YA5050) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Rtn-3.

Host

Mouse

Molecular Weight

Predicted band size: 113kDa;

Species Reactivity

Human

SwissProt ID

O95197

Gene ID

10313 [NCBI]

Immunogen

Purified recombinant fragment of RTN3 expressed in E.Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. Induces the formation of endoplasmic reticulum tubules (PubMed:25612671). Also acts as an inflammation-resolving regulator by interacting with both TRIM25 and RIGI, subsequently impairing RIGI 'Lys-63'-linked polyubiquitination leading to IRF3 and NF-kappa-B inhibition; (Microbial infection) Plays a positive role in viral replication and pathogenesis of enteroviruses

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Isomer 3 is widely expressed, with the highest concentration in the brain, particularly enriched in the cell bodies of gray matter neurons (protein level) . Its concentration in the macula is three times that in the peripheral retina. Isomer 1 is highly expressed in the brain and poorly expressed in skeletal muscle. Isomer 2 is found only in melanoma.

Induction:By endoplasmic reticulum stress (at protein level) (PubMed:17191123) . Up-regulated and self-aggregates upon RNA viral infection (PubMed:34313226)

Isoforms & Post-Translational Modification：O95197 has 7 isomers: O95197-1: 112611 Da (predicted); O95197-2: 110652 Da (predicted); O95197-3: 25609 Da (predicted); O95197-4: 27568 Da (predicted); O95197-5: 26452 Da (predicted); O95197-6: 23489 Da (predicted); O95197-7: 100824 Da (predicted).

Subunit：Homodimer. Interacts with ATL1 (By similarity). Interacts with RTN4. Isoform 3 interacts with BACE1, BACE2, BCL2 and FADD. Interacts with ATL2. Interacts with TMEM33 (By similarity). Interacts with ZFYVE27 and with KIF5A in a ZFYVE27-dependent manner (PubMed:21976701). Interacts with RIGI (PubMed:34313226). Interacts with TRIM25 (PubMed:34313226)

Synonyms

RTN3; ASYIP; NSPL2; Reticulon-3; Neuroendocrine-specific protein-like 2; NSP-like protein 2; Neuroendocrine-specific protein-like II; NSP-like protein II; NSPLII

Documentation

User Guide for Antibodies (1077 KB)