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Sodium Potassium ATPase Antibody (YA5555)

(Synonyms: Sodium/potassium-transporting ATPase subunit alpha-1; Na; +; /K; +; ATPase alpha-1 subunit; Sodium pump subunit alpha-1; )
Customer Review

Based on 1 Customer Validation

Sodium Potassium ATPase Antibody (YA5555) is a Mouse-derived and non-conjugated IgG3 monoclonal antibody, targeting to Sodium Potassium ATPase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Sodium Potassium ATPase Antibody (YA5555) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:100-500 1:1000-5000

Product Details

Description

Sodium Potassium ATPase Antibody (YA5555) is a Mouse-derived and non-conjugated IgG3 monoclonal antibody, targeting to Sodium Potassium ATPase.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
Immunogen

Synthesized peptide derived from human Sodium Potassium ATPtase AA range: 150-250

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    Immunohistochemical analysis of paraffin-embedded human kidney using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    Immunohistochemical analysis of paraffin-embedded rat small intestine using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    Immunohistochemical analysis of paraffin-embedded human pancreas using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    Immunohistochemical analysis of paraffin-embedded human colon using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Sodium Potassium ATPase is a P05023: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis
    P13637: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients
    P50993: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients
    Q13733: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility
    [1][2].

  • Subcellular Localization

    P05023: Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Cell membrane, sarcolemma; Multi-pass membrane protein; Cell projection, axon; Melanosome
    P13637: Cell membrane; Multi-pass membrane protein
    P50993: Membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein
    Q13733: Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity: Q13733: Specifically expressed in testis. Found in very low levels in skeletal muscle. Expressed in mature sperm (at protein level)

  • Subunit

    P05023: Interacts with regulatory subunit FXYD3 (PubMed:21454534) .Binds the HLA class II histocompatibility antigen DR1 (PubMed:1380674). Interacts with SLC35G1 and STIM1 (PubMed:22084111).
    P13637: The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1
    P50993: The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1
    Q13733: The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit

  • SwissProt ID

    P05023 / P50993 / P13637 / Q13733

  • Gene ID
    476 [NCBI] / 477 [NCBI] / 478 [NCBI] / 480 [NCBI]
  • Synonyms

    Sodium/potassium-transporting ATPase subunit alpha-1; Na; +; /K; +; ATPase alpha-1 subunit; Sodium pump subunit alpha-1;

Sodium Potassium ATPase Antibody (YA5555) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat small intestine using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Sodium Potassium ATPase Antibody (YA5555) Product Image
    1/16
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Sodium Potassium ATPase Antibody (YA5555) Product Image
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat small intestine using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Experimental Validation Results for Sodium Potassium ATPase Antibody (YA5555)
  • Sodium Potassium ATPase Antibody (YA5555) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Sodium Potassium ATPase Antibody (YA5555) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat small intestine using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Sodium Potassium ATPase Antibody (YA5555) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Sodium Potassium ATPase Antibody (YA5555) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon using Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P845863, 1/1000) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Sodium Potassium ATPase Antibody (YA5555) Product Image IHC-P
  • Sodium Potassium ATPase Antibody (YA5555) Product Image IHC-P
  • Sodium Potassium ATPase Antibody (YA5555) Product Image IHC-P
  • Sodium Potassium ATPase Antibody (YA5555) Product Image IHC-P