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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. TCF3 / E2A Antibody (YA1220)

TCF3 / E2A Antibody (YA1220)

Cat. No.: HY-P81475
User Guide for Antibodies Technical Support

TCF3 / E2A Antibody (YA1220) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF3 / E2A.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Descripciòn

Descripciòn

TCF3 / E2A Antibody (YA1220) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF3 / E2A.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 67.6 kDa;
Observed band size: 67 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human TCF-3
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:1000-1:2000
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Background
Function：Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed:2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity); Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity
Subcellular Localization：Nucleus
Isoforms & Post-Translational Modification：P15923 has 3 isomers: P15923-1: 67600 Da (predicted); P15923-2: 67265 Da (predicted); P15923-3: 68510 Da (predicted).
Phosphorylated following NGF stimulation;Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes
Subunit：Homodimer (PubMed:14752053, PubMed:2112746). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein (By similarity). Forms a heterodimer with ASH1, TWIST1 and TWIST2 (By similarity). Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities (By similarity). Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element (PubMed:14752053). Forms a heterodimer with TCF15; the heterodimer binds E-box element (By similarity). Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity). Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 (By similarity). Interacts with NEUROD2, PTF1A and TGFB1I1 (By similarity). Interacts with EP300 and UBE2I (PubMed:14752053, PubMed:9409784). Interacts with BHLHA9 (PubMed:25466284). Interacts with ASB2; the interaction is mediated by SKP2 and targets TCF3 for Notch-induced proteasomal degradation (PubMed:21119685)
Database

Entrez Gene: 6929 Human

SwissProt: P15923 Human

OMIM: 616941 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
TCF3; BHLHB21, E2A, ITF1; Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21 (bHLHb21); Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3 (TCF-3); Transcription factor ITF-1
Documentación
User Guide for Antibodies (1077 KB)

TCF3 / E2A Antibody (YA1220) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
TCF3 / E2A Antibody (YA1220)
Cat. No.:
HY-P81475
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