TOLLIP Antibody (YA7789)
(Synonyms: IL-1RAcPIP)
Based on 1 Customer Validation
TOLLIP Antibody (YA7789) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TOLLIP.
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Host:
Mouse
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Isotype:
IgG1
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Application:
ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|
| Dilution Ratio | 1:100-250 |
Product Details
TOLLIP Antibody (YA7789) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TOLLIP.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 30.1 kDa
Full length human recombinant protein of human TOLLIP produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TOLLIP component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P)[1][2][3][4][5].
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Subcellular Localization
Cytoplasm,Endosome,Early endosome
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Isoforms & Post-Translational Modification
Q9H0E2 has two isomers: Q9H0E2-1: 30282 Da (predicted); Q9H0E2-2: 22812 Da (predicted).
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products -
Subunit
Oligomerizes (PubMed:11751856)
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SwissProt ID
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Synonyms
IL-1RAcPIP
Documentation
References
[1]. Burns K, et al. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nat Cell Biol. 2000 Jun;2(6):346-51. [Content Brief]
[2]. Zhang G, et al. Negative regulation of toll-like receptor-mediated signaling by Tollip. J Biol Chem. 2002 Mar 1;277(9):7059-65. [Content Brief]
[3]. Lu K, et al. Autophagic clearance of polyQ proteins mediated by ubiquitin-Atg8 adaptors of the conserved CUET protein family. Cell. 2014 Jul 31;158(3):549-63. [Content Brief]
[4]. Katoh Y, et al. Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes. J Biol Chem. 2004 Jun 4;279(23):24435-43. [Content Brief]
[5]. Xiao S, et al. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure. 2015 Oct 6;23(10):1910-1920. [Content Brief]
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