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VISTA Antibody (YA1276)

(Synonyms: VSIR; PP2135, UNQ730/PRO1412; C10orf54, SISP1; VISTA; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; Stress-induced secreted protein-1; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor)
Customer Review

Based on 1 Customer Validation

VISTA Antibody (YA1276) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VISTA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • VISTA Antibody (YA1276) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000

Product Details

Description

VISTA Antibody (YA1276) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VISTA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45-60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Species Reactivity Database

Entrez Gene: 64115 Human

SwissProt: Q9H7M9 Human

Immunogen

Recombinant protein of human VISTA

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VISTA Antibody (YA1276)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for VISTA Antibody (YA1276)
    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for VISTA Antibody (YA1276)
    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    V-domain immunoglobulin suppressor of T cell activation (VISTA) is a novel inhibitory immune checkpoints (ICs) that is expressed on myeloid cells, lymphoid cells, and tumoral cells; therefore, VISTA can substantially regulate innate and

    adaptive anti-tumoral immune responses. VISTA is a transmembrane protein that plays a crucial role in the regulation of antitumor immunity. Therapeutic intervention that inhibits the VISTA pathway constitutes a new approach in the treatment of cancer.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level) . Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea. Has low expression levels in other tissues

  • SwissProt ID

    Q9H7M9

  • Gene ID
    64115 [NCBI]
  • Synonyms

    VSIR; PP2135, UNQ730/PRO1412; C10orf54, SISP1; VISTA; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; Stress-induced secreted protein-1; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor

  • Research Field

    Cardiovascular

References

[1]. Ait Boujmia OK. V-domain Ig suppressor of T cell activation (VISTA) inhibition is a new approach to cancer therapy: a Bibliometric study. Naunyn Schmiedebergs Arch Pharmacol. 2021 Jun;394(6):1057-1065.

[2]. Hosseinkhani N, et al. The Role of V-Domain Ig Suppressor of T Cell Activation (VISTA) in Cancer Therapy: Lessons Learned and the Road Ahead. Front Immunol. 2021 May 19;12:676181.

VISTA Antibody (YA1276) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • VISTA Antibody (YA1276) Product Image
    1/16
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • VISTA Antibody (YA1276) Product Image
  • Experimental Validation Results for VISTA Antibody (YA1276)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for VISTA Antibody (YA1276)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for VISTA Antibody (YA1276)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • Experimental Validation Results for VISTA Antibody (YA1276)
  • VISTA Antibody (YA1276) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • VISTA Antibody (YA1276) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • VISTA Antibody (YA1276) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using VISTA antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P81531, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • VISTA Antibody (YA1276) Product Image IHC-P
  • VISTA Antibody (YA1276) Product Image IHC-P
  • VISTA Antibody (YA1276) Product Image IHC-P