Benzyldodecyldimethylammonium chloride dihydrate

Name: Benzyldodecyldimethylammonium chloride dihydrate
Brand: MedChemExpress
SKU: HY-128384
Rating: 4.5 (1 reviews)

Cat. No.: HY-128384 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Benzyldodecyldimethylammonium chloride dihydrate is a quaternary ammonium compound (QAC) and can be used as a biocide to target antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant (MDR) P. aeruginosa et. al. Benzyldodecyldimethylammonium chloride dihydrate, an antimicrobial agent, bacteriostatic or bactericidal properties depending on the concentration.

For research use only. We do not sell to patients.

Benzyldodecyldimethylammonium chloride dihydrate Chemical Structure

CAS No. : 147228-80-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

MIC: 20 mg/L (P. fluorescens)^[1]

In Vitro

Benzyldodecyldimethylammonium chloride dihydrate (1-10 μM; 2 hours) results in a dose-dependent changes in the percent population of dead rat thymocyte cells^[1].
Benzyldodecyldimethylammonium chloride dihydrate (0-40 mg/L; 0-24 hours) inhibits P. fluorescens with a MIC of 20 mg/L. At 5 mg/L, cells have the same growth behaviour as the control. At concentrations of 10 and 15 mg/L the growth profile is different from the control, and the cells start to grow only after 8 h of adaptation. At concentrations of ≥20 mg/L, BDMDAC inhibits cell growth^[2].
Benzyldodecyldimethylammonium chloride dihydrate interacts strongly with cell surfaces in a concentration-dependent manner, and binds by ionic and hydrophobic interactions to microbial membrane surfaces, as manifested by phenomena such as membrane disruption and loss of membrane integrity with consequent leakage of essential intracellular constituents, which promote significant and irreversible changes in the cell structure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Rat thymocyte cells
Concentration:	0, 5, 10, 15, 20 and 40 mg/L
Incubation Time:	2 hours
Result:	Resulted in cell death.

Cell Proliferation Assay^[1]

Cell Line:	Bacterial P. fluorescen
Concentration:	0, 5, 10, 15, 20 and 40 mg/L
Incubation Time:	2 hours
Result:	Inhibited bacterial growth in a time and dose dependent manner.

Molecular Weight

376.02

Formula

C₂₁H₄₂ClNO₂

CAS No.

147228-80-6

Appearance

Solid

Color

White to off-white

SMILES

C[N+](C)(CCCCCCCCCCCC)CC1=CC=CC=C1.[Cl-].O.O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (265.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (265.94 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6594 mL	13.2972 mL	26.5943 mL
5 mM	0.5319 mL	2.6594 mL	5.3189 mL
10 mM	0.2659 mL	1.3297 mL	2.6594 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (699 KB)

COA (243 KB) HNMR (252 KB)

Handling Instructions (2659 KB)

References

[1]. Sadegh Ghanbar, et al. New Generation of N-Chloramine/QAC Composite Biocides: Efficient Antimicrobial Agents To Target Antibiotic-Resistant Bacteria in the Presence of Organic Load. ACS Omega. 2018 Aug 22;3(8):9699-9709. [Content Brief]

[2]. Tsuyoshi Mitani, et al. Zinc-related actions of sublethal levels of benzalkonium chloride: Potentiation of benzalkonium cytotoxicity by zinc. Chem Biol Interact. 2017 Apr 25;268:31-36. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.6594 mL	13.2972 mL	26.5943 mL	66.4858 mL
	5 mM	0.5319 mL	2.6594 mL	5.3189 mL	13.2972 mL
	10 mM	0.2659 mL	1.3297 mL	2.6594 mL	6.6486 mL
	15 mM	0.1773 mL	0.8865 mL	1.7730 mL	4.4324 mL
	20 mM	0.1330 mL	0.6649 mL	1.3297 mL	3.3243 mL
	25 mM	0.1064 mL	0.5319 mL	1.0638 mL	2.6594 mL
	30 mM	0.0886 mL	0.4432 mL	0.8865 mL	2.2162 mL
	40 mM	0.0665 mL	0.3324 mL	0.6649 mL	1.6621 mL
	50 mM	0.0532 mL	0.2659 mL	0.5319 mL	1.3297 mL
	60 mM	0.0443 mL	0.2216 mL	0.4432 mL	1.1081 mL
	80 mM	0.0332 mL	0.1662 mL	0.3324 mL	0.8311 mL
	100 mM	0.0266 mL	0.1330 mL	0.2659 mL	0.6649 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.