Benzyldodecyldimethylammonium chloride dihydrate
Based on 1 Customer Validation
Benzyldodecyldimethylammonium chloride dihydrate is a quaternary ammonium compound (QAC) and can be used as a biocide to target antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant (MDR) P. aeruginosa et. al. Benzyldodecyldimethylammonium chloride dihydrate, an antimicrobial agent, bacteriostatic or bactericidal properties depending on the concentration.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 147228-80-6
- Formula: C21H42ClNO2
- Molecular Weight:376.02
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Tetraethylammonium bromide
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HY-D0172
Tetrabutylammonium bromide
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Tetraethylammonium chloride
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Benzethonium chloride (Standard)
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HY-W133907
Tetrakis(hydroxymethyl)phosphonium sulfate
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HY-W111436
Tetrabutylammonium tetraphenylborate
Biological Activity
MIC: 20 mg/L (P. fluorescens)[1]
Benzyldodecyldimethylammonium chloride dihydrate (1-10 μM; 2 hours) results in a dose-dependent changes in the percent population of dead rat thymocyte cells[1].Benzyldodecyldimethylammonium chloride dihydrate (0-40 mg/L; 0-24 hours) inhibits P. fluorescens with a MIC of 20 mg/L. At 5 mg/L, cells have the same growth behaviour as the control. At concentrations of 10 and 15 mg/L the growth profile is different from the control, and the cells start to grow only after 8 h of adaptation. At concentrations of ≥20 mg/L, BDMDAC inhibits cell growth[2].Benzyldodecyldimethylammonium chloride dihydrate interacts strongly with cell surfaces in a concentration-dependent manner, and binds by ionic and hydrophobic interactions to microbial membrane surfaces, as manifested by phenomena such as membrane disruption and loss of membrane integrity with consequent leakage of essential intracellular constituents, which promote significant and irreversible changes in the cell structure[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat thymocyte cells
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Concentration:0, 5, 10, 15, 20 and 40 mg/L
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Incubation Time:2 hours
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Result:Resulted in cell death.
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Cell Line:Bacterial P. fluorescen
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Concentration:0, 5, 10, 15, 20 and 40 mg/L
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Incubation Time:2 hours
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Result:Inhibited bacterial growth in a time and dose dependent manner.
Chemical Information
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CAS No. 147228-80-6
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Appearance Solid
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Molecular Weight 376.02
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Formula C21H42ClNO2
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Color White to off-white
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SMILES
C[N+](C)(CCCCCCCCCCCC)CC1=CC=CC=C1.[Cl-].O.O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (265.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (265.94 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (759 KB)
- English - EN (759 KB)
- Français - FR (759 KB)
- Deutsch - DE (759 KB)
- Norwegian - NO (759 KB)
- Español - ES (759 KB)
- Swedish - SV (759 KB)
- Italian - IT (759 KB)
- Korean - KR (759 KB)
- Portuguese - PT (759 KB)
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Handling Instructions (2659 KB)
References
[1]. Sadegh Ghanbar, et al. New Generation of N-Chloramine/QAC Composite Biocides: Efficient Antimicrobial Agents To Target Antibiotic-Resistant Bacteria in the Presence of Organic Load. ACS Omega. 2018 Aug 22;3(8):9699-9709. [Content Brief]
[2]. Tsuyoshi Mitani, et al. Zinc-related actions of sublethal levels of benzalkonium chloride: Potentiation of benzalkonium cytotoxicity by zinc. Chem Biol Interact. 2017 Apr 25;268:31-36. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.6594 mL | 13.2972 mL | 26.5943 mL | 66.4858 mL |
| 5 mM | 0.5319 mL | 2.6594 mL | 5.3189 mL | 13.2972 mL | |
| 10 mM | 0.2659 mL | 1.3297 mL | 2.6594 mL | 6.6486 mL | |
| 15 mM | 0.1773 mL | 0.8865 mL | 1.7730 mL | 4.4324 mL | |
| 20 mM | 0.1330 mL | 0.6649 mL | 1.3297 mL | 3.3243 mL | |
| 25 mM | 0.1064 mL | 0.5319 mL | 1.0638 mL | 2.6594 mL | |
| 30 mM | 0.0886 mL | 0.4432 mL | 0.8865 mL | 2.2162 mL | |
| 40 mM | 0.0665 mL | 0.3324 mL | 0.6649 mL | 1.6621 mL | |
| 50 mM | 0.0532 mL | 0.2659 mL | 0.5319 mL | 1.3297 mL | |
| 60 mM | 0.0443 mL | 0.2216 mL | 0.4432 mL | 1.1081 mL | |
| 80 mM | 0.0332 mL | 0.1662 mL | 0.3324 mL | 0.8311 mL | |
| 100 mM | 0.0266 mL | 0.1330 mL | 0.2659 mL | 0.6649 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Benzyldodecyldimethylammonium chloride dihydrate Related Classifications
HY-128384 Related Classifications
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