Cat. No.: HY-B0942

Benzethonium chloride

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Benzethonium chloride inhibits nicotinic acetylcholine receptors in human recombinant α7 and α4β2 neurons in Xenopus laevis oocytes, which has antibacterial, anticancer, antisepsis and disinfection activity. Benzethonium chloride induced Apoptosis and activated caspases in cancer cell lines. Benzethonium chloride ablates the tumor-forming ability of FaDu cells, delays the growth of xenograft tumors in vivo.

For research use only. We do not sell to patients.

Purity: 98.47%
CAS No.: 121-54-0
Formula: C₂₇H₄₂ClNO₂
Molecular Weight:448.08

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Benzethonium chloride Chemical Structure

CAS No.: 121-54-0

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	3.2 μM Compound: BztCl	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23792315]
CCRF-CEM	IC₅₀	3.8 μM Compound: BztCl	Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 23792315]
HeLa	IC₅₀	5.7 μM Compound: BztCl	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 23792315]
HUVEC	IC₅₀	47 μM Compound: BztCl	Cytotoxicity against HUVEC after 72 hrs by MTT assay Cytotoxicity against HUVEC after 72 hrs by MTT assay	[PMID: 23792315]
MCF7	IC₅₀	5 μM Compound: BztCl	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 23792315]
MDA-MB-231	IC₅₀	4.8 μM Compound: BztCl	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 23792315]
Vero	EC₅₀	0.01 μg/mL Compound: hyamine	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 21546254]

In Vitro

Benzethonium chloride (0.1 g/L, 10 s) results in only a 50% reduction in bacterial number against S. mutans MT8148 and Streptococcus sobrinus^[1].
Benzethonium chloride (0.1-100 μM, 48 h) reduces cell viability against FaDu and C666-1 (both human cancer) cell lines with EC₅₀ values of 3.8 and 5.3 μM, respectively^[2].
Benzethonium chloride (9 μM, 24-48 h) induces apoptosis and caspase activation in FaDu cells^[2].
Benzethonium chloride (0.1 and 5 mg/L, 24-72 h) shows great acute toxicity to C. elegans, induces an inhibition on hatching and increases mortality in zebrafish embryos^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:

FaDu cells
Concentration:

9 μM
Incubation Time:

24-48 h
Result:

Revealed nuclear condensation and blebbing and Caspase-2, caspase-8, and caspase-9 activations, indicative of apoptosis, after 48 hours of treatment.

In Vivo

Benzethonium chloride (5 mg/kg, i.p., daily for 5 days) results in elimination of tumor formation and growth delay in established xenograft tumors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Established xenograft tumors of mice^[2]
Dosage:

5 mg/kg
Administration:

i.p., daily for 5 days
Result:

Effectively eliminates the tumorforming potential of FaDu cells and survived for 3.5 days more than PBS-treated mice, which was a statistically significant delay.

Chemical Information

CAS No. 121-54-0
Appearance Solid
Molecular Weight 448.08
Formula C₂₇H₄₂ClNO₂
Color White to off-white
SMILES

CC(C)(C)CC(C1=CC=C(OCCOCC[N+](C)(C)CC2=CC=CC=C2)C=C1)(C)C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (1)

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Journal Impact Factor

Pathol Res Pract

Lactobacillus casei serves as a primary functional strain in fecal microbiota-mediated neuroprotection against cerebral ischemia/reperfusion injury. [Abstract]

2026 Jun:282:156441. PMID: 41946028

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (74.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (44.63 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2317 mL

11.1587 mL

22.3174 mL

5 mM

0.4463 mL

2.2317 mL

4.4635 mL

10 mM

0.2232 mL

1.1159 mL

2.2317 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 120 mg/mL (267.81 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (785 KB)
- English - EN (785 KB)
- Français - FR (785 KB)
- Deutsch - DE (785 KB)
- Norwegian - NO (785 KB)
- Español - ES (785 KB)
- Swedish - SV (785 KB)
- Italian - IT (785 KB)
- Korean - KR (785 KB)
- Portuguese - PT (785 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Maillard JY. Impact of benzalkonium chloride, benzethonium chloride and chloroxylenol on bacterial antimicrobial resistance. J Appl Microbiol. 2022 Dec;133(6):3322-3346. [Content Brief]

[2]. Yip KW, et al. Benzethonium chloride: a novel anticancer agent identified by using a cell-based small-molecule screen. Clin Cancer Res. 2006 Sep 15;12(18):5557-69. [Content Brief]

[3]. Sreevidya VS, et al. Benzalkonium chloride, benzethonium chloride, and chloroxylenol - Three replacement antimicrobials are more toxic than triclosan and triclocarban in two model organisms. Environ Pollut. 2018 Apr;235:814-824. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.2317 mL	11.1587 mL	22.3174 mL	55.7936 mL
	5 mM	0.4463 mL	2.2317 mL	4.4635 mL	11.1587 mL
	10 mM	0.2232 mL	1.1159 mL	2.2317 mL	5.5794 mL
	15 mM	0.1488 mL	0.7439 mL	1.4878 mL	3.7196 mL
	20 mM	0.1116 mL	0.5579 mL	1.1159 mL	2.7897 mL
	25 mM	0.0893 mL	0.4463 mL	0.8927 mL	2.2317 mL
	30 mM	0.0744 mL	0.3720 mL	0.7439 mL	1.8598 mL
	40 mM	0.0558 mL	0.2790 mL	0.5579 mL	1.3948 mL
DMSO	50 mM	0.0446 mL	0.2232 mL	0.4463 mL	1.1159 mL
DMSO	60 mM	0.0372 mL	0.1860 mL	0.3720 mL	0.9299 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Benzethonium chloride

1 Cited Publications

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Benzethonium chloride

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Publications Citing Use of MedChemExpress (MCE) Benzethonium chloride

All Caspase Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Benzethonium chloride Related Classifications

HY-B0942 Related Classifications