Cat. No.: HY-B2151

Digitoxigenin

(Synonyms: Cerberigenin; Echujetin)

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Based on 1 Customer Validation

Digitoxigenin is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 143-62-4
Formula: C₂₃H₃₄O₄
Molecular Weight:374.51

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Digitoxigenin Chemical Structure

CAS No.: 143-62-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Digitoxigenin is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.24 μg/mL Compound: 17	Cytotoxicity against human A549 cells after 3 days by MTT assay Cytotoxicity against human A549 cells after 3 days by MTT assay	[PMID: 16252914]
A549	IC₅₀	>316 μM Compound: 5	Viability of human A549 cells by MTT assay after 24 hrs Viability of human A549 cells by MTT assay after 24 hrs	[PMID: 17595134]
A549	IC₅₀	0.62 μM Compound: 5	Inhibition of induction of ICAM1 expression in human A549 cells in presence of IL1-alpha after 1 hr Inhibition of induction of ICAM1 expression in human A549 cells in presence of IL1-alpha after 1 hr	[PMID: 17595134]
A549	IC₅₀	1.68 μM Compound: DGTN	Cytotoxicity against human A549 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human A549 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
BC	IC₅₀	8.95 μM Compound: 3	Cytotoxicity against human BC cells by SRB assay Cytotoxicity against human BC cells by SRB assay	[PMID: 16933890]
BC	IC₅₀	8.95 x 10^-3 M Compound: 3	Cytotoxicity against human BC cells by SRB assay Cytotoxicity against human BC cells by SRB assay	[PMID: 16933890]
HCT-8	IC₅₀	2.48 μM Compound: DGTN	Cytotoxicity against human HCT8 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human HCT8 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
HepG2	IC₅₀	0.12 μg/mL Compound: 17	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 16252914]
HepG2	IC₅₀	18 mM Compound: 5	Growth inhibition of human HepG2 cells Growth inhibition of human HepG2 cells	[PMID: 17595134]
Hs-578T	IC₅₀	1.022 μM Compound: digitoxigenin	Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
Hs-578T	IC₅₀	92.3 μM Compound: digitoxigenin	Cytotoxicity against human Hs578T cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control Cytotoxicity against human Hs578T cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control	[PMID: 22316168]
HT-1080	IC₅₀	1500 nM Compound: 12	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 14640513]
KB	IC₅₀	9.61 μM Compound: 3	Cytotoxicity against human KB cells by SRB assay Cytotoxicity against human KB cells by SRB assay	[PMID: 16933890]
KB	IC₅₀	9.61 x 10^-3 M Compound: 3	Cytotoxicity against human KB cells by SRB assay Cytotoxicity against human KB cells by SRB assay	[PMID: 16933890]
LNCaP	IC₅₀	3.7 μM Compound: DGTN	Cytotoxicity against human LNCAP cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human LNCAP cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
MCF7	IC₅₀	1.69 μg/mL Compound: 17	Cytotoxicity against human MCF7 cells after 3 days by MTT assay Cytotoxicity against human MCF7 cells after 3 days by MTT assay	[PMID: 16252914]
MCF7	IC₅₀	194.4 nM Compound: 2	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 16309315]
MCF7	IC₅₀	0.6 μM Compound: 4, DTG	Antiproliferative activity against human MCF7 cells assessed as cell viability treated for 48 hrs followed by compound washout measured after 72 hrs by fluorometric CellTiter-Blue reagent assay Antiproliferative activity against human MCF7 cells assessed as cell viability treated for 48 hrs followed by compound washout measured after 72 hrs by fluorometric CellTiter-Blue reagent assay	[PMID: 21421322]
MCF7	IC₅₀	9.1 μM Compound: digitoxigenin	Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
MDA-MB-231	IC₅₀	1.9 μg/mL Compound: 17	Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay	[PMID: 16252914]
MRC5	IC₅₀	1.16 μM Compound: DGTN	Cytotoxicity against human MRC5 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
NCI-H187	IC₅₀	9.77 μM Compound: 3	Cytotoxicity against human NCI-H187 cells by SRB assay Cytotoxicity against human NCI-H187 cells by SRB assay	[PMID: 16933890]
NCI-H187	IC₅₀	9.77 x 10^-3 M Compound: 3	Cytotoxicity against human NCI-H187 cells by SRB assay Cytotoxicity against human NCI-H187 cells by SRB assay	[PMID: 16933890]
NCI-H460	IC₅₀	0.92 μM Compound: DGTN	Cytotoxicity against human H460 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human H460 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
PC-3	IC₅₀	2.41 μM Compound: DGTN	Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
SK-BR-3	IC₅₀	4.42 μM Compound: digitoxigenin	Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
T47D	IC₅₀	2.53 μM Compound: digitoxigenin	Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
TK-10	IC₅₀	76.5 nM Compound: 2	Cytotoxicity against human TK10 cells after 48 hrs by SRB assay Cytotoxicity against human TK10 cells after 48 hrs by SRB assay	[PMID: 16309315]
UACC-62	IC₅₀	348.8 nM Compound: 2	Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay	[PMID: 16309315]
Vero	CC₅₀	27.54 μM Compound: DGTN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 30798081]
Vero	IC₅₀	1.02 μM Compound: DGTN	Antiviral activity against acyclovir-resistant Herpes simplex virus 1 29-R infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay Antiviral activity against acyclovir-resistant Herpes simplex virus 1 29-R infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay	[PMID: 30798081]
Vero	IC₅₀	1.09 μM Compound: DGTN	Antiviral activity against acyclovir-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay Antiviral activity against acyclovir-sensitive Herpes simplex virus 1 KOS infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay	[PMID: 30798081]
Vero	IC₅₀	3.23 μM Compound: DGTN	Antiviral activity against Herpes simplex virus 2-333 infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay Antiviral activity against Herpes simplex virus 2-333 infected in African green monkey Vero cells assessed as inhibition of viral replication after 48 hrs by naphthol blue-black staining-based plaque reduction assay	[PMID: 30798081]
WI-38	IC₅₀	11.8 mM Compound: 5	Growth inhibition of human WI38 cells Growth inhibition of human WI38 cells	[PMID: 17595134]
WI-38 VA13	IC₅₀	1.9 mM Compound: 5	Growth inhibition of human VA13 cells Growth inhibition of human VA13 cells	[PMID: 17595134]

Chemical Information

CAS No. 143-62-4
Appearance Solid
Molecular Weight 374.51
Formula C₂₃H₃₄O₄
Color Off-white to light yellow
SMILES

O[C@@]12[C@@]3([H])[C@@](CC[C@@]1([C@@H](C(CO4)=CC4=O)CC2)C)([H])[C@@]5([C@](C[C@H](CC5)O)([H])CC3)C
Synonyms

Cerberigenin; Echujetin
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (333.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6702 mL

13.3508 mL

26.7016 mL

5 mM

0.5340 mL

2.6702 mL

5.3403 mL

10 mM

0.2670 mL

1.3351 mL

2.6702 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (245 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6702 mL	13.3508 mL	26.7016 mL	66.7539 mL
	5 mM	0.5340 mL	2.6702 mL	5.3403 mL	13.3508 mL
	10 mM	0.2670 mL	1.3351 mL	2.6702 mL	6.6754 mL
	15 mM	0.1780 mL	0.8901 mL	1.7801 mL	4.4503 mL
	20 mM	0.1335 mL	0.6675 mL	1.3351 mL	3.3377 mL
	25 mM	0.1068 mL	0.5340 mL	1.0681 mL	2.6702 mL
	30 mM	0.0890 mL	0.4450 mL	0.8901 mL	2.2251 mL
	40 mM	0.0668 mL	0.3338 mL	0.6675 mL	1.6688 mL
	50 mM	0.0534 mL	0.2670 mL	0.5340 mL	1.3351 mL
	60 mM	0.0445 mL	0.2225 mL	0.4450 mL	1.1126 mL
	80 mM	0.0334 mL	0.1669 mL	0.3338 mL	0.8344 mL
	100 mM	0.0267 mL	0.1335 mL	0.2670 mL	0.6675 mL

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Digitoxigenin

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Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

Help & FAQs

Complete Stock Solution Preparation Table

Digitoxigenin

Natural Product Library

Medicine Food Homology Compound Library

Traditional Chinese Medicine Active Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Tibetan Medicine Compound Library

Protein Kinase Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

Help & FAQs

Complete Stock Solution Preparation Table

Digitoxigenin Related Classifications

HY-B2151 Related Classifications