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Cat. No.: HY-123225

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LCRF-0004

LCRF-0004 is a potent inhibitor of RON kinase, with the IC₅₀ value of 10 nM. and a surprising result is also obtained regarding its c-Met inhibitory activity, with the IC₅₀ value of 12 nM. LCRF-0004 plays an important role in cancer research.

For research use only. We do not sell to patients.

CAS No.: 1229611-73-7
Formula: C₂₈H₁₈F₄N₆O₂S
Molecular Weight:578.54

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

LCRF-0004 Chemical Structure

CAS No.: 1229611-73-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.1 μM Compound: LCRF-0004	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 25953155]
A549	IC₅₀	0.4 μM Compound: 4	Inhibition of c-Met-mediated cell migration of human A549 cells by would healing Inhibition of c-Met-mediated cell migration of human A549 cells by would healing	[PMID: 20363625]
DU-145	IC₅₀	2 μM Compound: 4	Inhibition of c-Met-mediated scattering in HGF-stimulated human DU145 cells Inhibition of c-Met-mediated scattering in HGF-stimulated human DU145 cells	[PMID: 20363625]
HCT-116	IC₅₀	0.08 μM Compound: 4	Antitumor activity against human HCT116 cells by MTT assay Antitumor activity against human HCT116 cells by MTT assay	[PMID: 20363625]
HCT-116	IC₅₀	0.08 μM Compound: LCRF-0004	Antiproliferative activity against human HCT116 cells by MTT assay Antiproliferative activity against human HCT116 cells by MTT assay	[PMID: 25953155]
HEK293	IC₅₀	0.05 μM Compound: 4	Inhibition of TPR-tagged RON catalytic domain expressed in HEK293 cells Inhibition of TPR-tagged RON catalytic domain expressed in HEK293 cells	[PMID: 20363625]
HT-29	IC₅₀	30 nM Compound: LCRF-0004	Inhibition of RON phosphorylation in human HT29 cells Inhibition of RON phosphorylation in human HT29 cells	[PMID: 25953155]
MDA-MB-231	IC₅₀	0.1 μM Compound: LCRF-0004	Antiproliferative activity against human MDA-MB-231 cells by MTT assay Antiproliferative activity against human MDA-MB-231 cells by MTT assay	[PMID: 25953155]
MKN-45	IC₅₀	0.5 μM Compound: 4	Inhibition of c-Met phosphorylation in human MKN45 cells Inhibition of c-Met phosphorylation in human MKN45 cells	[PMID: 20363625]
MKN-45	IC₅₀	6.87 μM Compound: LCRF-0004	Inhibition of phosphorylation of c-Met in human MKN45 cells Inhibition of phosphorylation of c-Met in human MKN45 cells	[PMID: 25953155]
MKN-45	IC₅₀	6.87 μM Compound: LCRF-0004	Inhibition of c-Met phosphorylation in human MKN45 cells Inhibition of c-Met phosphorylation in human MKN45 cells	[PMID: 26243370]
PC-3	IC₅₀	30 nM Compound: LCRF-0004	Inhibition of RON phosphorylation in human PC3 cells Inhibition of RON phosphorylation in human PC3 cells	[PMID: 25953155]

Chemical Information

CAS No. 1229611-73-7
Molecular Weight 578.54
Formula C₂₈H₁₈F₄N₆O₂S
SMILES

O=C(NC1=CC=C(C(F)=C1)OC2=C3C(C=C(S3)C4=CN(C=N4)C)=NC=C2)C5=C(N(N=C5)C6=CC=CC=C6)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Stéphane L Raeppel, et al. Design and synthesis of close analogs of LCRF-0004, a potent and selective RON receptor tyrosine kinase inhibitor. Bioorg Med Chem Lett. 2015 Jun 15;25(12):2527-31. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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