Azide-PEG5-Ms
Azide-PEG5-Ms serves as a PEG linker containing an azide that reacts with BCN, DBCO, alkyne via Click Chemistry and mesyl group that is a good leaving group for nucleophilic substitution reactions. The PEG spacer imparts water solubility.
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- CAS No.: 940951-97-3
- Formule: C11H23N3O7S
- Masse moléculaire:341.38
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
Chemical Information
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CAS No. 940951-97-3
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Masse moléculaire 341.38
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Formule C11H23N3O7S
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SMILES
[N-]=[N+]=NCCOCCOCCOCCOCCOS(=O)(C)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)