Guanylyltransferase
Guanylyltransferase (GTase) plays a central role in the three-step catalytic process of adding an m7GpppN cap cotranscriptionally to nascent mRNA (pre-mRNAs).
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- CAS No.: 593278-77-4
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
Chemical Information
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CAS No. 593278-77-4
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SMILES
[Guanylyltransferase]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Pureté et documentation
Références
[1]. Garg G, et al. Structural insights into human co-transcriptional capping. Mol Cell. 2023 Jul 20;83(14):2464-2477.e5. [Content Brief]
[2]. Chu C, et al. Structure of the guanylyltransferase domain of human mRNA capping enzyme. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10104-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Guanylyltransferase Related Classifications
HY-E70405 Related Classifications
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