Criblage à haut débit par SPRi

Surface Plasmon Resonance Imaging (SPRi) high-throughput screening is a label-free, real-time parallel optical sensing technique for biomolecular interaction analysis. It upgrades the single-point detection of conventional SPR to microarray imaging, enabling simultaneous analysis of hundreds to thousands of molecular interactions in a single run, which drastically boosts the efficiency of drug screening and molecular mechanism research. This technology is ideally suited for early-stage large-scale drug screening and fragment screening. It effectively shortens R&D cycles, cuts research costs, and improves the quality of candidate compounds.

Technical Advantages

Ultra-high throughput: Simultaneously characterize interactions between thousands of compounds and target proteins per assay. Throughput is over 400 times higher than conventional SPR, making it ideal for screening large compound libraries.
Label-free detection: No fluorescent or isotopic labeling required, eliminating structural and functional perturbations induced by labeling.
Real-time kinetic profiling: Captures the full binding process in real time: association → equilibrium → dissociation.
Multidimensional kinetic readouts: Directly generates kinetic parameters including kon (association rate constant), koff (dissociation rate constant) and Kd (equilibrium dissociation constant), supporting structure-activity relationship (SAR) analysis, compound prioritization and mechanism-of-action validation.

Experimental Workflow

  • 1
    Preparation of microarray chip: Select compatible chip and densely print thousands of small-molecule compounds or samples on the solid surface to prepare microarrays.
  • 2
    Sample Injection: Inject target proteins into the flow cell. Analytes flow across the chip surface and bind immobilized small molecules for real-time tracking of binding events.
  • 3
    Data Acquisition: Real-time tracking of SPR signal shifts. Full association-dissociation profiles are recorded to produce sensorgrams per array spot.
  • 4
    Data Analysis: Fit sensorgrams from each spot to calculate binding kinetic parameters. Rank compounds by affinity to filter potent hits against target proteins.

Service Advantages

• One-stop drug discovery platform delivering integrated solutions spanning hit identification & validation through lead compound optimization and synthesis to meet diverse research and development demands.
• Experienced technical team with extensive project expertise, offering screening services for small molecules, peptides, nucleic acids and other biomolecules.
• Rigorous standardized data privacy management to secure your research throughout the project.

Online Consulting

SPRi High Throughput Screening Service requires technical evaluation to confirm customized protocols and pricing. For detailed pricing or technical information, please email [email protected] or contact sales representatives directly.

Product Customization

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