2. GPCR/G Protein Neuronal Signaling
  3. Dopamine Receptor mAChR Adrenergic Receptor 5-HT Receptor
  4. Clozapine hydrochloride

Clozapine hydrochloride  (Synonyms: HF 1854 hydrochloride)

Cat. No.: HY-14539A Purity: 99.83%
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Clozapine hydrochloride (HF 1854 hydrochloride) is an antipsychotic used for the research of schizophrenia. Clozapine hydrochloride has high affinity for a number of neuroreceptors. Clozapine hydrochloride is a potent antagonist of dopamine D2 with a Ki of 75 nM. Clozapine hydrochloride inhibits the muscarinic M1 receptor and serotonin 5HT2A receptor with Kis of 9.5 nM and 4 nM, respectively. Clozapine hydrochloride is also a potent and selective agonist at the muscarinic M4 receptor (EC50=11 nM).

For research use only. We do not sell to patients.

CAS No. : 54241-01-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
25 mg In-stock
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Based on 11 publication(s) in Google Scholar

Other Forms of Clozapine hydrochloride:

Clozapine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Clozapine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 13;16(1):11318.  [Abstract]

    In isoflurane anesthetized mice, the injection of Clozapine (0.03 mg/kg; i.p.) significantly increased EEG-based arousal index, but from a low to middle arousal level.

    Clozapine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 1;15(1):7603.  [Abstract]

    Representative current clamp recording of a NPYDRN/vlPAG-hM4Di neuron with the bath of Clozapine (5 μM). Average membrane potential and firing frequency before and after Clozapine application to inhibit NPY DRN/vlPAGneurons were also recorded. Clozapine effectively inhibited hM4Di+ NPYDRN/vlPAG neurons with decreased neuronal membrane potential and firing frequency.

    Clozapine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 1;15(1):7603.  [Abstract]

    Percentage of body weight changes, daily food intake and its area under curve (AUC) of mCherry-restraint and hM4Di-restraint mice. Clozapine (i.p.; 0.2mg/kg; 30 min before restraint for 14 d) caused NPYhM4Di mice to consume less food and lost more body weight than NPYmCherry mice during chronic restraint stress.

    Clozapine hydrochloride purchased from MedChemExpress. Usage Cited in: FEBS J. 2024 Nov;291(21):4680-4695.  [Abstract]

    HaCaT cells were transfected with siNC or siRNA against TRPM7 and then treated with Clozapine (Clo) (10 μM; 6 d) the next day. Clozapine (Clo) promoted activation of TRPM7 and increased SA-b-Gal activity.

    Clozapine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Psychiatry. 2021 Jun;26(6):2514-2532.  [Abstract]

    The administration of Clozapine (i.p.; 2 d) restored impaired PPI in female ZFP804A mutant mice.

    View All Dopamine Receptor Isoform Specific Products:

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Clozapine hydrochloride (HF 1854 hydrochloride) is an antipsychotic used for the research of schizophrenia. Clozapine hydrochloride has high affinity for a number of neuroreceptors. Clozapine hydrochloride is a potent antagonist of dopamine D2 with a Ki of 75 nM. Clozapine hydrochloride inhibits the muscarinic M1 receptor and serotonin 5HT2A receptor with Kis of 9.5 nM and 4 nM, respectively[1][2][3]. Clozapine hydrochloride is also a potent and selective agonist at the muscarinic M4 receptor (EC50=11 nM)[4].

    IC50 & Target

    5-HT2A Receptor

    4 nM (Ki)

    5-HT2A Receptor

     

    5-HT6 Receptor

     

    Human 5-HT7 Receptor

     

    mAChR1

    9.5 nM (Ki)

    mAChR4

    11 nM (EC50)

    α2-adrenergic receptor

    51 nM (Ki)

    D2 Receptor

    75 nM (Ki)

    In Vivo

    Clozapine hydrochloride (25 mg/kg/day; i.p.; 21 days) shows antipsychotic effects in lysergic acid diethylamide-induced psychosis mouse model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male 129 S6/Sv mice, lysergic acid diethylamide (LSD)-induced psychosis model[3]
    Dosage: 25 mg/kg/day
    Administration: Intraperitoneal injection, 21 days
    Result: Decreased head-twitch response, reduced 5-HT2A mRNA, rescued induction of c-fos, but not egr-1 and egr-2.
    Clinical Trial
    Molecular Weight

    363.28

    Formula

    C18H20Cl2N4
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    CN1CCN(C2=NC3=CC(Cl)=CC=C3NC4=CC=CC=C42)CC1.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (275.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7527 mL 13.7635 mL 27.5270 mL
    5 mM 0.5505 mL 2.7527 mL 5.5054 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7527 mL 13.7635 mL 27.5270 mL 68.8174 mL
    5 mM 0.5505 mL 2.7527 mL 5.5054 mL 13.7635 mL
    10 mM 0.2753 mL 1.3763 mL 2.7527 mL 6.8817 mL
    15 mM 0.1835 mL 0.9176 mL 1.8351 mL 4.5878 mL
    20 mM 0.1376 mL 0.6882 mL 1.3763 mL 3.4409 mL
    25 mM 0.1101 mL 0.5505 mL 1.1011 mL 2.7527 mL
    30 mM 0.0918 mL 0.4588 mL 0.9176 mL 2.2939 mL
    40 mM 0.0688 mL 0.3441 mL 0.6882 mL 1.7204 mL
    50 mM 0.0551 mL 0.2753 mL 0.5505 mL 1.3763 mL
    60 mM 0.0459 mL 0.2294 mL 0.4588 mL 1.1470 mL
    80 mM 0.0344 mL 0.1720 mL 0.3441 mL 0.8602 mL
    100 mM 0.0275 mL 0.1376 mL 0.2753 mL 0.6882 mL
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    Clozapine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Clozapine54241-01-9HF 1854HF1854HF-1854Dopamine ReceptormAChRAdrenergic Receptor5-HT ReceptorMuscarinic acetylcholine receptorBeta ReceptorSerotonin Receptor5-hydroxytryptamine ReceptorantipsychoticschizophreniaHeLapsychosisInhibitorinhibitorinhibit

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