Columbamine chloride
Based on 1 publication(s) in Google Scholar
Columbamine (Columbamin; Dehydroisocorypalmine) chloride is an organic heterotetracyclic alkaloid extracted from plants. Columbamine chloride is a metabolite of Berberine (HY-N0716). Columbamine chloride inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine chloride induces apoptosis in cancer cells. Columbamine chloride can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 1916-10-5
- Formula: C20H20ClNO4
- Molecular Weight:373.83
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Columbamine chloride
MoreAll Parasite Isoforms
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Biological Activity
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CYP3A4 30.6 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
77.9 μM
Compound: Columbamine chloride
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Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
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[PMID: 11141105] |
Columbamine (M2) (15 μM, 24 h) chloride decreases the intracellular triglyceride (TG) contents in Hep G2 cells[2].
Columbamine (15 μM, 24 h) chloride induces a reduction of lipid synthesis and increases in fatty acid oxidation mediated via AMPK activation in Hep G2 cells[2].
Columbamine (10-50 μM, 24-72 h) chloride inhibits the proliferation of colon cancer cells via suppressing Wnt/β-catenin signaling pathway[4].
Columbamine (20-40 μM, 24 h) chloride inhibits the invasion and migration of colon cancer cells[4].
Columbamine (20-40 μM, 48 h) chloride triggers apoptosis by inhibiting a caspase-dependent mechanism in colon cancer cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LoVo, HCT-116, SW480 (Human colorectal cancer cell lines)
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Concentration:10, 20, 30, 40, 50 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited the growth of the colon cells, which were proportional to treatment time and dosage.
Suppressed clone formation of HCT116 cells in a concentration and time-dependent manner.
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Cell Line:LoVo, HCT-116
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Concentration:20, 30, 40 μM
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Incubation Time:24 h
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Result:Significantly inhibited the migration and invasion of HCT116 and Lovo cells compared with the control cells.
Greatly impaired the invasion and migration of HCT116 cells in dose-dependent manner.
Suppressed the expression of N-Cadherin and promoted the expression of E-cadherin, resulting in the inhibition of epithelial-mesenchymal transition (EMT) of colon cancer cells.
Attenuated expressions of MMP2, MMP7 and MMP9 proteins.
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Cell Line:HCT-116
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Concentration:20, 30, 40 μM
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Incubation Time:48 h
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Result:Significantly prevented cell growth and promoted cell death at concentration from 20 μM to 40 μM.
Increased the population of Annexin V-positive cells.
The cleaved form of caspase-3 and PARP were increased without affecting the expression of caspase-3 and PARP.
Promoted pro-apoptosis factor BAD expression and repressed anti-apoptosis factor Bcl-2 expression.
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Cell Line:Hep G2 cells
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Concentration:15 μM
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Incubation Time:24 h
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Result:Inhibited the expression of genes, such as FAS, HMGR, ACC and GPAT, which are associated with fat synthesis.
Increased the gene expression of mCAD, but had no effects on CPT-1.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Six-weeks-old male BALB/c nude mice (subcutaneously injected with HCT116 and control cells on the opposite flanks of the same mouse)[4]
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Dosage:5, 10, 20 mg/kg
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Administration:Administered to experimental mice for 23 days
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Result:Decreased the tumor volumes in a dose-dependent manner.
Chemical Information
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CAS No. 1916-10-5
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Appearance Solid
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Molecular Weight 373.83
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Formula C20H20ClNO4
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Color Light yellow to yellow
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SMILES
COC1=C(OC)C2=C[N+]3=C(C4=CC(O)=C(OC)C=C4CC3)C=C2C=C1.[Cl-]
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Synonyms
Columbamin chloride; Dehydroisocorypalmine chloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Molecules
Discovery of Nine Dipeptidyl Peptidase-4 Inhibitors from Coptis chinensis Using Virtual Screening, Bioactivity Evaluation, and Binding Studies. [Abstract]2024 May 14;29(10):2304. PMID: 38792165
Solvent & Solubility
DMSO : 5 mg/mL (13.38 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang W, et al. A series of strategies for solving the shortage of reference standards for multi-components determination of traditional Chinese medicine, Mahoniae Caulis as a case. J Chromatogr A. 2015 Sep 18;1412:100-11. [Content Brief]
[2]. Wright CW, et al. In vitro antiplasmodial, antiamoebic, and cytotoxic activities of some monomeric isoquinoline alkaloids. J Nat Prod. 2000 Dec;63(12):1638-40. [Content Brief]
[3]. Cao S, et al. Berberine metabolites exhibit triglyceride-lowering effects via activation of AMP-activated protein kinase in Hep G2 cells. J Ethnopharmacol. 2013 Sep 16;149(2):576-82. [Content Brief]
[4]. Su CR, et al. Cytochrome P3A4 inhibitors and other constituents of Fibraurea tinctoria. J Nat Prod. 2007 Dec;70(12):1930-3. [Content Brief]
[5]. ei C, et al. Columbamine suppresses the proliferation and malignization of colon cancer cells via abolishing Wnt/β-catenin signaling pathway. Cancer Manag Res. 2019 Sep 23;11:8635-8645. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6750 mL | 13.3751 mL | 26.7501 mL | 66.8753 mL |
| 5 mM | 0.5350 mL | 2.6750 mL | 5.3500 mL | 13.3751 mL | |
| 10 mM | 0.2675 mL | 1.3375 mL | 2.6750 mL | 6.6875 mL |
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