Cat. No.: HY-N1916

Coniferyl ferulate

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Coniferyl ferulate is an orally active phenolic acid compound. Coniferyl ferulate is a potent inhibitor of glutathione S-transferase (GST) (IC₅₀ = 0.3 μM), which downregulates P-gp expression, induces apoptosis in B-MD-C1 (ADR+/+) cells, and reverses multidrug resistance. Coniferyl ferulate blocks the NMDAR/NR2B-CaMKII-MAPKs signaling pathway, inhibits ROS production and mitochondrial apoptosis, while reshapes the intestinal microbiota and microbial metabolism, ameliorates colonic inflammation and alleviates depressive symptoms in mice. Coniferyl ferulate can alleviate the toxicity of xylene to hematopoietic stem and progenitor cells by targeting Mgst2. Coniferyl ferulate exhibits antibacterial activity against the Gram-positive Bacillus subtilis and Staphylococcus aureus.

For research use only. We do not sell to patients.

Purity: 98.73%
CAS No.: 63644-62-2
Formula: C₂₀H₂₀O₆
Molecular Weight:356.37

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Coniferyl ferulate Chemical Structure

CAS No.: 63644-62-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]

CaMK II

NMDA Receptor

All iGluR Isoforms :

All CaMK Isoforms :

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	IC₅₀	19.8 μM Compound: 40; coniferyl ferulate	Antimigratory activity against human MDA-MB-231 cells expressing c-MET assessed as inhibition of HGF-induced cell migration incubated for 24 hrs by Giemsa staining based wound healing assay Antimigratory activity against human MDA-MB-231 cells expressing c-MET assessed as inhibition of HGF-induced cell migration incubated for 24 hrs by Giemsa staining based wound healing assay	[PMID: 27258622]
MDA-MB-231	IC₅₀	22.3 μM Compound: 40; coniferyl ferulate	Antiproliferative activity against human MDA-MB-231 cells expressing c-MET assessed as inhibition of HGF-induced cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells expressing c-MET assessed as inhibition of HGF-induced cell growth incubated for 72 hrs by MTT assay	[PMID: 27258622]
MDA-MB-468	IC₅₀	25.6 μM Compound: 40; coniferyl ferulate	Antiproliferative activity against human MDA-MB-468 cells expressing c-MET assessed as inhibition of HGF-induced cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells expressing c-MET assessed as inhibition of HGF-induced cell growth incubated for 72 hrs by MTT assay	[PMID: 27258622]

In Vitro

Coniferyl ferulate (5-20 μM, 48 h) induces apoptosis and markedly inhibits cells in G1-phase in B-MD-C1 (ADR+/+) cells^[1].
Coniferyl ferulate (5-20 μM, 48 h) inhibits the upregulation of MDR1/P-gp caused by Doxorubicin (HY-15142A), and bring the expression of P-gp close to the basal level in B-MD-C1 (ADR+/+) cells^[1].
Coniferyl ferulate (0.2-20 μM, 28 h) protects against the decline in cell viability and apoptosis induced by Glutamate (HY-14608), and alleviates cell membrane damage by blocking the NMDA receptor-CaMKII-MAPKs signaling cascade, reducing the level of ROS and enhancing SOD activity in PC12 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

B-MD-C1(ADR+/+) cells
Concentration:

5, 10 and 20 μM
Incubation Time:

48 h
Result:

Induced apoptosis.

Cell Cycle Analysis^[1]

Cell Line:

B-MD-C1(ADR+/+) cells
Concentration:

5, 10 and 20 μM
Incubation Time:

48 h
Result:

Markedly increased the proportion of cells in G1-phase, decreased the proportion of cells in S-phase.

RT-PCR^[1]

Cell Line:

B-MD-C1(ADR+/+) cells
Concentration:

5, 10 and 20 μM
Incubation Time:

48 h
Result:

Dose-dependently inhibited Doxorubicin-induced MDR1 overexpression.
Brought the expression of P-gp close to the basal level.

Cell Viability Assay^[2]

Cell Line:

Glutamate-damaged PC12 cells
Concentration:

0.2, 2, 20 μM
Incubation Time:

After 4 hours of pre-treatment, with Glutamate for another 24 hours
Result:

Concentration-dependently protected the decline in cell viability induced by Glutamate.
Significantly alleviated the damage to the cell membrane caused by Glutamate.

Apoptosis Analysis^[2]

Cell Line:

Glutamate-damaged PC12 cells
Concentration:

0.2, 2, 20 μM
Incubation Time:

After 4 hours of pre-treatment, with Glutamate for another 24 hours
Result:

Reduced nuclear shrinkage and PI-positive cells.

Apoptosis Analysis^[2]

Cell Line:

Glutamate-damaged PC12 cells
Concentration:

0.2, 2, 20 μM
Incubation Time:

After 4 hours of pre-treatment, with Glutamate for another 24 hours
Result:

Decreased the level of p-NR2B, p-CaMKII, p-JNK and p-p38.
Increased the Bcl-2/Bax ratio and decreased the release of cytochrome C and activation of Caspase-3.

In Vivo

Coniferyl ferulate (25-100 mg/kg, i.g., once daily, for 13-14 days) possesses anti-depressant effect in mice behavioral test^[2].
Coniferyl ferulate (50 mg/kg, i.g., once daily, for 4 weeks) attenuates depression-like and anxiety-like behaviors induced by chronic unpredicted mild stress (CUMS), and significantly ameliorates colonic inflammation in mice^[3].
Coniferyl ferulate (50 mg/kg, i.g., once daily, for 25 days) alleviates xylene-caused hematopoietic stem and progenitor cell toxicity by inhibiting Mgst2 in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Behavioral test (tail suspension and forced-swimming test) established in male ICR mice (18-22 g)^[2]
Dosage:

25, 50 and 100 mg/kg
Administration:

Intragastric administration (i.g.), once daily, for 13 and 14 days
Result:

Obviously decreased the immobility time in tail suspension test (TST) and forced swimming test (FST).

Animal Model:

CUMS model established in in 8-week-old male C57BL/6 SPF mice (20 g)^[3]
Dosage:

50 mg/kg
Administration:

Intragastric administration (i.g.), once daily, for 4 weeks
Result:

Improved depressive in sucrose preference test (SPT), open field test (OFT), FTS and TST and anxiety-like behaviors in elevated plus-maze test (EPM).
Restored the reduction of intestinal microvilli and alleviated mitochondrial swelling.
Lowered the levels of IL-6, IL-1β, and TNF-α.
Restructured the gut microbiome, and microbial metabolism.

Animal Model:

Xylene-induced hematotoxicity model established in male C57BL/6 SPF mice (8 weeks old, 20 g)
Dosage:

50 mg/kg
Administration:

Intragastric administration (i.g.), once daily, for 25 days
Result:

Significantly reversed the White blood cell count (WBC) reduction induced by xylene.
Restored the proportion of bone marrow LSK cells to the normal level.
Reduced the level of ROS in mitochondria and restored the mitochondrial membrane potential inhibited by xylene.
Acted directly on the Mgst2 target site (K_D = 8.0861 kcal/mol).

Chemical Information

CAS No. 63644-62-2
Appearance Solid
Molecular Weight 356.37
Formula C₂₀H₂₀O₆
Color White to off-white
SMILES

O=C(OC/C=C/C1=CC=C(O)C(OC)=C1)/C=C/C2=CC=C(O)C(OC)=C2
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Polyphenols
Initial Source
- Plants
- Umbelliferae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

Am J Respir Cell Mol Biol

MGST2hi PMNs mediate NETs and drive pericyte-to-myofibroblast transition in RA-ILD. [Abstract]

2026 Mar 25:aanag065. PMID: 42089332

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (280.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8061 mL

14.0304 mL

28.0607 mL

5 mM

0.5612 mL

2.8061 mL

5.6121 mL

10 mM

0.2806 mL

1.4030 mL

2.8061 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (7.02 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen C, et al. Coniferyl Ferulate, a Strong Inhibitor of Glutathione S-Transferase Isolated from Radix Angelicae sinensis, Reverses Multidrug Resistance and Downregulates P-Glycoprotein. Evid Based Complement Alternat Med. 2013;2013:639083. [Content Brief]

[2]. Gong W, et al. Coniferyl ferulate exerts antidepressant effect via inhibiting the activation of NMDAR-CaMKII-MAPKs and mitochondrial apoptotic pathways. J Ethnopharmacol. 2020 Apr 6;251:112533. [Content Brief]

[3]. Hao WZ, et al. Oral coniferyl ferulate attenuated depression symptoms in mice via reshaping gut microbiota and microbial metabolism. Food Funct. 2021 Dec 13;12(24):12550-12564. [Content Brief]

[4]. Yin Z, et al. Coniferyl ferulate alleviate xylene-caused hematopoietic stem and progenitor cell toxicity by Mgst2. Front Pharmacol. 2024 Mar 8;15:1334445. [Content Brief]

[5]. Chou SC, et al. Antibacterial activity of components from Lomatium californicum. Phytother Res. 2006 Feb;20(2):153-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8061 mL	14.0304 mL	28.0607 mL	70.1518 mL
	5 mM	0.5612 mL	2.8061 mL	5.6121 mL	14.0304 mL
	10 mM	0.2806 mL	1.4030 mL	2.8061 mL	7.0152 mL
	15 mM	0.1871 mL	0.9354 mL	1.8707 mL	4.6768 mL
	20 mM	0.1403 mL	0.7015 mL	1.4030 mL	3.5076 mL
	25 mM	0.1122 mL	0.5612 mL	1.1224 mL	2.8061 mL
	30 mM	0.0935 mL	0.4677 mL	0.9354 mL	2.3384 mL
	40 mM	0.0702 mL	0.3508 mL	0.7015 mL	1.7538 mL
	50 mM	0.0561 mL	0.2806 mL	0.5612 mL	1.4030 mL
	60 mM	0.0468 mL	0.2338 mL	0.4677 mL	1.1692 mL
	80 mM	0.0351 mL	0.1754 mL	0.3508 mL	0.8769 mL
	100 mM	0.0281 mL	0.1403 mL	0.2806 mL	0.7015 mL

Keywords

Coniferyl ferulate

1 Cited Publications

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Coniferyl ferulate

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Publications Citing Use of MedChemExpress (MCE) Coniferyl ferulate

All iGluR Isoforms

All CaMK Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation