Cross-linked dextran G 50

Cross-linked dextran G 50 can be used for isolation and purification of protected peptides with certain molecule range.
Methods for Cross-linked dextran G 50 instructment:
1. Gel swelling: Swell the G-50 gel in a hexamethylphosphoramide (HMPA) solvent containing 5% water at 50°C for 16 hours.
2. Degassing: Degas the swollen gel to remove dissolved air.
3. Column loading: Load the degassed G-50 gel into the chromatographic column with an inner diameter of 2.5 cm or 5.0 cm.
4. Sample loading: Load the sample to be purified onto the packed G-50 column.
5. Elution: Elute the chromatographic column with HMPA-5% aqueous solution and elute at a flow rate of 7.5 mL/h or 20 mL/h. Monitor the UV absorbance of the eluent at 280 nm.
6. Collect: Collect the eluent of the target peptide for subsequent purifications.
7. Remove HMPA: Since HMPA cannot be removed by evaporation, the peptide can be separated from HMPA by gel filtration using a G-10 gel column and DMF solvent.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.