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DSG3 Protein, Human (sf9, His)

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Based on 1 Customer Validation

DSG3 is a component of intercellular desmosome junctions and plays a key role in promoting intercellular adhesion by participating in important interactions with plaque proteins and intermediate filaments. Carefully orchestrated interactions between DSG3 and these structural elements are critical for maintaining the integrity and strength of cell-to-cell connections. DSG3 Protein, Human (sf9, His) is the recombinant human-derived DSG3 protein, expressed by sf9 insect cells , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DSG3 Protein, Human (sf9, His)
  • Experimental Validation Results for DSG3 Protein, Human (sf9, His)
  • DSG3 Protein, Human (sf9, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

DSG3 is a component of intercellular desmosome junctions and plays a key role in promoting intercellular adhesion by participating in important interactions with plaque proteins and intermediate filaments. Carefully orchestrated interactions between DSG3 and these structural elements are critical for maintaining the integrity and strength of cell-to-cell connections. DSG3 Protein, Human (sf9, His) is the recombinant human-derived DSG3 protein, expressed by sf9 insect cells , with C-6*His labeled tag.

Background

Desmoglein-3 (DSG3) is an integral component of intercellular desmosome junctions, playing a crucial role in mediating cell-cell adhesion. Functioning within these junctions, DSG3 is involved in the interaction between plaque proteins and intermediate filaments, contributing to the structural integrity and stability of tissues. Notably, DSG3 interacts with Plakophilin-2 (PKP2), emphasizing its role in coordinating the assembly and maintenance of desmosomal complexes. This interaction further highlights the intricate molecular network involved in cell-cell adhesion and reinforces the importance of DSG3 in ensuring the proper function and structural cohesion of tissues through its engagement in desmosomal signaling pathways. (

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC50 is <5.720 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 2.736 ng/mL.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-6*His
  • Accession

    P32926 (E50-R615)

  • Gene ID
    1830  [NCBI]
  • Molecular Construction
    • N-term
    • DSG3 (E50-R615)
      Accession # P32926
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    DSG3; Desmoglein-3; Desmoglein 3; PVA; CDHF6; Desmoglein 3 (Pemphigus Vulgaris Antigen); 130 KDa Pemphigus Vulgaris Antigen; Pemphigus Vulgaris Antigen; Cadherin Family Member 6; ABOLM

  • AA Sequence

    EWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR

  • Molecular Weight

    Approximately 74 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC50 is 2.736 ng/mL.

  • DSG3 Protein, Human (sf9, His) Product Image
    1/16
  • Experimental Validation Results for DSG3 Protein, Human (sf9, His)
  • Experimental Validation Results for DSG3 Protein, Human (sf9, His)
  • DSG3 Protein, Human (sf9, His) Product Image