DSG3 Protein, Human (sf9, His)

DSG3 is a component of intercellular desmosome junctions and plays a key role in promoting intercellular adhesion by participating in important interactions with plaque proteins and intermediate filaments. Carefully orchestrated interactions between DSG3 and these structural elements are critical for maintaining the integrity and strength of cell-to-cell connections. DSG3 Protein, Human (sf9, His) is the recombinant human-derived DSG3 protein, expressed by sf9 insect cells , with C-6*His labeled tag.

Desmoglein-3 (DSG3) is an integral component of intercellular desmosome junctions, playing a crucial role in mediating cell-cell adhesion. Functioning within these junctions, DSG3 is involved in the interaction between plaque proteins and intermediate filaments, contributing to the structural integrity and stability of tissues. Notably, DSG3 interacts with Plakophilin-2 (PKP2), emphasizing its role in coordinating the assembly and maintenance of desmosomal complexes. This interaction further highlights the intricate molecular network involved in cell-cell adhesion and reinforces the importance of DSG3 in ensuring the proper function and structural cohesion of tissues through its engagement in desmosomal signaling pathways. (

Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC₅₀ is <5.720 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody, the EC₅₀ is 2.736 ng/mL.

Human

Sf9 insect cells

C-6*His

P32926 (E50-R615)

1830  [NCBI]

Extracellular Domain

EWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR

Approximately 74 kDa, due to glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/hepg2-cell.html  [Content Brief]