FABP4 Protein, Human (His)

FABP4 Protein, expressed mainly in adipocytes, crucially mediates lipid and retinoic acid transport. It binds long-chain fatty acids and retinoic acid, facilitating delivery to nuclear receptors. Functioning as a monomer and forming homodimers, FABP4 interacts with PPARG, contributing to its involvement in fatty acid metabolism and signaling pathways. FABP4 Protein, Human (His) is the recombinant human-derived FABP4 protein, expressed by E. coli , with N-6*His labeled tag.

FABP4, a lipid transport protein predominantly expressed in adipocytes, serves as a crucial mediator in lipid and retinoic acid transport. Its role involves binding to both long-chain fatty acids and retinoic acid, facilitating their delivery to respective receptors within the nucleus. FABP4 functions as a monomer and can form homodimers. Additionally, it engages in interactions with PPARG, contributing to its involvement in key processes related to fatty acid metabolism and signaling pathways.

Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 65.15 ng/mL, corresponding to a specific activity is 1.535×10^4 units/mg.

• 
  Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 65.15 ng/mL, corresponding to a specific activity is 1.535×10^4 units/mg.

Human

E. coli

N-6*His

P15090 (C2-A132)

2167  [NCBI]

Full Length of Mature Protein

CDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA

Approximately 16 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/mda-mb-231-cell.html  [Content Brief]