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MAdCAM1 Protein, Mouse (HEK293, His)

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MAdCAM1 is expressed in mucosal venules and serves as a key cell adhesion leukocyte receptor that guides the influx of lymphocytes into mucosal tissues. It regulates leukocyte passage and retention, effectively binding to integrins α-4/β-7 and L-selectin to achieve comprehensive leukocyte adhesion control. MAdCAM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAdCAM1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

MAdCAM1 is expressed in mucosal venules and serves as a key cell adhesion leukocyte receptor that guides the influx of lymphocytes into mucosal tissues. It regulates leukocyte passage and retention, effectively binding to integrins α-4/β-7 and L-selectin to achieve comprehensive leukocyte adhesion control. MAdCAM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MAdCAM1 protein, expressed by HEK293 , with C-His labeled tag.

Background

MAdCAM1 protein, expressed by mucosal venules, serves as a cell adhesion leukocyte receptor crucial for directing lymphocyte traffic into mucosal tissues, including the Peyer patches and the intestinal lamina propria. This protein plays a pivotal role in regulating both the passage and retention of leukocytes. MAdCAM1 can effectively bind to both integrin alpha-4/beta-7 and L-selectin, facilitating comprehensive control over leukocyte adhesion. Both isoform 1 and isoform 2 of MAdCAM1 demonstrate the ability to adhere to integrin alpha-4/beta-7. Notably, isoform 2, distinguished by its lack of the mucin-like domain, may specialize in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independently of L-selectin binding. The protein forms homodimers to accomplish its functional roles in mediating cell adhesion.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q61826-1 (Q22-S364)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • MAdCAM1 (Q22-S364)
      Accession # Q61826-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MADCAM1; HMAdCAM-1; Mucosal Vascular Addressin Cell Adhesion Molecule 1; MAdCAM-1; Mucosal Addressin Cell Adhesion Molecule 1; Mucosal Addressin Cell Adhesion Molecule-1; MACAM1

  • AA Sequence

    QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGEVTNLYVPGQVTPNSSS

  • Predicted Molecular Mass

    32.5 kDa

  • Molecular Weight

    Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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