VCAM-1/CD106 Protein, Mouse (HEK293, His)

VCAM-1 protein is a member of the immunoglobulin superfamily.It can act as a regulator of capillary formation and liver fibrosis, and can promote liver fibrosis.VCAM-1 Protein plays an important role in immune response and inflammatory response.VCAM-1/CD106 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-His labeled tag.

VCAM-1 protein supports leukocyte adhesion by binding to α4β1 integrin and is crucial for the recruitment of monocytes and lymphocytes during inflammation^[1][5].
VCAM-1 Protein is a regulator of capillary formation and hepatic fibrosis in liver sinusoidal endothelial cells (LSECs). VCAM-1 Protein can activate the Yes-associated protein 1 pathway and induce fibrosis phenotypes in HSCs^[2].
VCAM-1 Protein causes persistent inflammation by inhibiting effective T cell apoptosis^[3].
VCAM-1 protein causes persistent inflammation by inhibiting the apoptosis of effector T cells^[3].
VCAM-1 protein plays an important role in regulating immune response and inflammatory response to viral infection^[4].
VCAM-1 Protein is necessary for smooth muscle cell migration. Targeting VCAM-1 expression can effectively prevent and treat atherosclerosis and restenosis^[5].

Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells.

Mouse

HEK293

C-His

Q3UPN1 (F25-E698)

22329  [NCBI]

Partial

FKIEISPEYKTIAQIGDSMALTCSTTGCESPLFSWRTQIDSPLNAKVRTEGSKSVLTMEPVSFENEHSYLCTATCGSGKLERSIHVDIYSFPKDPEIQFSGPLEVGKPVTVKCLAPDIYPVYRLEIDLFKGDQLMNRQEFSSEEMTKSLETKSLEVTFTPVIEDIGKALVCRAKLHIDQIDSTLKERETVKELQVYISPRNTTISVHPSTRLQEGGAVTMTCSSEGLPAPEIFWGRKLDNEVLQLLSGNATLTLIAMRMEDSGVYVCEGVNLIGRDKAEVELVVQEKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSFSWRTQTDSPLNGVVRNEGAKSTLVLSSVGFEDEHSYLCAVTCLQRTLEKRTQVEVYSFPEDPVIKMSGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLMKKYFLEEMGIKSLETKILETTFIPTIEDTGKSLVCLARLHSGEMESEPKQRQSVQPLYVNVAPKETTIWVSPSPILEEGSPVNLTCSSDGIPAPKILWSRQLNNGELQPLSENTTLTFMSTKRDDSGIYVCEGINEAGISRKSVELIIQVSPKDIQLTVFPSKSVKEGDTVIISCTCGNVPETWIILKKKAKTGDMVLKSVDGSYTIRQAQLQDAGIYECESKTEVGSQLRSLTLDVKGKEHNKNYFSPE

Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Chung KJ, et al. Analysis of the Role of Stellate Cell VCAM-1 in NASH Models in Mice. Int J Mol Sci. 2023 Mar 2;24(5):4813.  [Content Brief]

  [2]. Guo, Qianqian et al. Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis. Frontiers in immunology vol. 13 983255. 25 Aug. 2022  [Content Brief]

  [3]. Afford, Simon C et al. Vascular cell adhesion molecule 1 expression by biliary epithelium promotes persistence of inflammation by inhibiting effector T-cell apoptosis. Hepatology (Baltimore, Md.) vol. 59,5 (2014): 1932-43. doi:10.1002/hep.26965  [Content Brief]

  [4]. Ou, Rong et al. Regulation of immune response and inflammatory reactions against viral infection by VCAM-1. Journal of virology vol. 82,6 (2008): 2952-65.  [Content Brief]

  [5]. Petersen, Erik J et al. siRNA silencing reveals role of vascular cell adhesion molecule-1 in vascular smooth muscle cell migration. Atherosclerosis vol. 198,2 (2008): 301-6.  [Content Brief]