VCAM-1/CD106 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
VCAM-1 protein is a member of the immunoglobulin superfamily.It can act as a regulator of capillary formation and liver fibrosis, and can promote liver fibrosis.VCAM-1 Protein plays an important role in immune response and inflammatory response.VCAM-1/CD106 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
VCAM-1 protein is a member of the immunoglobulin superfamily.It can act as a regulator of capillary formation and liver fibrosis, and can promote liver fibrosis.VCAM-1 Protein plays an important role in immune response and inflammatory response.VCAM-1/CD106 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-His labeled tag.
Background
VCAM-1 protein supports leukocyte adhesion by binding to α4β1 integrin and is crucial for the recruitment of monocytes and lymphocytes during inflammation[1][5].
VCAM-1 Protein is a regulator of capillary formation and hepatic fibrosis in liver sinusoidal endothelial cells (LSECs). VCAM-1 Protein can activate the Yes-associated protein 1 pathway and induce fibrosis phenotypes in HSCs[2].
VCAM-1 Protein causes persistent inflammation by inhibiting effective T cell apoptosis[3].
VCAM-1 protein causes persistent inflammation by inhibiting the apoptosis of effector T cells[3].
VCAM-1 protein plays an important role in regulating immune response and inflammatory response to viral infection[4].
VCAM-1 Protein is necessary for smooth muscle cell migration. Targeting VCAM-1 expression can effectively prevent and treat atherosclerosis and restenosis[5].
Verified Bioactivity
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q3UPN1 (F25-E698)
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Molecular Construction
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N-term
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VCAM-1 (F25-E698)
Accession # Q3UPN1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
VCAM1; Vascular Cell Adhesion Molecule 1; Vascular Cell Adhesion Protein 1; CD106; CD106 Antigen; INCAM-100; V-CAM 1; VCAM-1
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AA Sequence
FKIEISPEYKTIAQIGDSMALTCSTTGCESPLFSWRTQIDSPLNAKVRTEGSKSVLTMEPVSFENEHSYLCTATCGSGKLERSIHVDIYSFPKDPEIQFSGPLEVGKPVTVKCLAPDIYPVYRLEIDLFKGDQLMNRQEFSSEEMTKSLETKSLEVTFTPVIEDIGKALVCRAKLHIDQIDSTLKERETVKELQVYISPRNTTISVHPSTRLQEGGAVTMTCSSEGLPAPEIFWGRKLDNEVLQLLSGNATLTLIAMRMEDSGVYVCEGVNLIGRDKAEVELVVQEKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSFSWRTQTDSPLNGVVRNEGAKSTLVLSSVGFEDEHSYLCAVTCLQRTLEKRTQVEVYSFPEDPVIKMSGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLMKKYFLEEMGIKSLETKILETTFIPTIEDTGKSLVCLARLHSGEMESEPKQRQSVQPLYVNVAPKETTIWVSPSPILEEGSPVNLTCSSDGIPAPKILWSRQLNNGELQPLSENTTLTFMSTKRDDSGIYVCEGINEAGISRKSVELIIQVSPKDIQLTVFPSKSVKEGDTVIISCTCGNVPETWIILKKKAKTGDMVLKSVDGSYTIRQAQLQDAGIYECESKTEVGSQLRSLTLDVKGKEHNKNYFSPE
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Predicted Molecular Mass
75.5 kDa
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Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Chung KJ, et al. Analysis of the Role of Stellate Cell VCAM-1 in NASH Models in Mice. Int J Mol Sci. 2023 Mar 2;24(5):4813. [Content Brief]
[2]. Guo, Qianqian et al. Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis. Frontiers in immunology vol. 13 983255. 25 Aug. 2022 [Content Brief]
[3]. Afford, Simon C et al. Vascular cell adhesion molecule 1 expression by biliary epithelium promotes persistence of inflammation by inhibiting effector T-cell apoptosis. Hepatology (Baltimore, Md.) vol. 59,5 (2014): 1932-43. doi:10.1002/hep.26965 [Content Brief]
[4]. Ou, Rong et al. Regulation of immune response and inflammatory reactions against viral infection by VCAM-1. Journal of virology vol. 82,6 (2008): 2952-65. [Content Brief]
[5]. Petersen, Erik J et al. siRNA silencing reveals role of vascular cell adhesion molecule-1 in vascular smooth muscle cell migration. Atherosclerosis vol. 198,2 (2008): 301-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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