P-Selectin Protein, Human (Biotinylated, HEK293, His-Avi)

P-selectin, a Ca(2+)-dependent receptor on myeloid cells, crucially binds to sialyl-Lewis X on neutrophils and monocytes, facilitating the interaction of activated endothelial cells or platelets with leukocytes. This engagement, primarily with SELPLG/PSGL1 and PODXL2, is vital for the rapid rolling of leukocytes during early inflammation. P-selectin's interactions with SNX17 further mediate neutrophil adhesion and rolling, dependent on the sialyl-Lewis X epitope and specific tyrosine sulfation on SELPLG. P-Selectin Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived P-Selectin protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of P-Selectin Protein, Human (Biotinylated, HEK293, His-Avi) is 730 a.a., with molecular weight of 115-140 kDa.

P-selectin, a Ca(2+)-dependent receptor on myeloid cells, facilitates the binding to carbohydrates on neutrophils and monocytes, playing a crucial role in the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized by P-selectin is sialyl-Lewis X, and this interaction is pivotal for the rapid rolling of leukocytes over vascular surfaces during the initial stages of inflammation, accomplished through engagement with SELPLG. Additionally, P-selectin forms interactions with SNX17 and mediates adhesion and rolling of neutrophils through its association with SELPLG/PSGL1 and PODXL2. This interaction is contingent on the sialyl-Lewis X epitope of SELPLG and PODXL2, as well as the specific tyrosine sulfation on SELPLG.

Measured by its binding ability in a functional ELISA. When immobilized Anti-P-selectin Antibody, hFc Tag at 0.5mg/ml (100μl/Well), can bind Biotinylated Human P-Selectin, His Tag and the EC₅₀ is 0.13 μg/mL.

Human

HEK293

C-Avi;C-His

P16109 (W42-A771)

6403  [NCBI]

115-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.