1. Apoptosis
  2. Bcl-2 Family Caspase Apoptosis
  3. Destruxin B

Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo.

For research use only. We do not sell to patients.

Destruxin B Chemical Structure

Destruxin B Chemical Structure

CAS No. : 2503-26-6

Size Price Stock Quantity
1 mg USD 780 In-stock
5 mg   Get quote  
10 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2].

IC50 & Target

Bcl-2

 

Caspase-3

 

In Vitro

Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1].
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1].
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2].
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: H1299 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Inhibited H1299 cells proliferation of H1299 cells with an IC50 of 4.1 μM.

Apoptosis Analysis[1]

Cell Line: Lung adenocarcinoma A549 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Induces caspase-dependent Lung adenocarcinoma A549 cells death.

Cell Viability Assay[2]

Cell Line: HT-29 human colorectal cancer cells
Concentration: 1.25, 2.50, 5.00, 10.00 and 20.00 μM
Incubation Time: 72 hours
Result: The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 10 or 20 μM
Incubation Time: 12 and 24  hours
Result: Regulates Mcl-1 and PUMA.
In Vivo

Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2]
Dosage: Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration: Injection; daily; for 6 weeks
Result: Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.
Molecular Weight

621.81

Formula

C32H55N5O7

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@]([C@@H](C)CC)(C)C(N(C)[C@@H](C(C)C)C(N(C)[C@@H](C)C(NCC1)=O)=O)=O)[C@@](CCC2)(C)N2C([C@@H](CC(C)C)OC1=O)=O

Structure Classification
Initial Source

Metarhizium anisopliae

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation

Purity: 99.86%

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Destruxin B Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Destruxin B
Cat. No.:
HY-N6690
Quantity:
MCE Japan Authorized Agent: