1. Apoptosis
  2. Bcl-2 Family
    Caspase
    Apoptosis
  3. Destruxin B

Destruxin B 

Cat. No.: HY-N6690
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Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo.

For research use only. We do not sell to patients.

Destruxin B Chemical Structure

Destruxin B Chemical Structure

CAS No. : 2503-26-6

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Description

Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2].

IC50 & Target

Bcl-2

 

Caspase-3

 

In Vitro

Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1].
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1].
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2].
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner [1].

Cell Proliferation Assay[1]

Cell Line: H1299 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Inhibited H1299 cells proliferation of H1299 cells with an IC50 of 4.1 μM.

Apoptosis Analysis[1]

Cell Line: Lung adenocarcinoma A549 cells
Concentration: 1, 5, 10, 20, 30 μM
Incubation Time: 48  hours
Result: Induces caspase-dependent Lung adenocarcinoma A549 cells death.

Cell Viability Assay[2]

Cell Line: HT-29 human colorectal cancer cells
Concentration: 1.25, 2.50, 5.00, 10.00 and 20.00 μM
Incubation Time: 72 hours
Result: The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 10 or 20 μM
Incubation Time: 12 and 24  hours
Result: Regulates Mcl-1 and PUMA.
In Vivo

Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2].

Animal Model: Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2]
Dosage: Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration: Injection; daily; for 6 weeks
Result: Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.
Molecular Weight

621.81

Formula

C₃₂H₅₅N₅O₇

CAS No.

2503-26-6

SMILES

O=C(N[[email protected]]([[email protected]@H](C)CC)(C)C(N(C)[[email protected]@H](C(C)C)C(N(C)[[email protected]@H](C)C(NCC1)=O)=O)=O)[[email protected]@](CCC2)(C)N2C([[email protected]@H](CC(C)C)OC1=O)=O

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Destruxin B
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