Protease Substrate

Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein.

N-Succinyl-Ile-Ile-Trp-AMC is a peptide-based fluorescent substrate or probe for carboxypeptidase Y (CPY).

4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant.

5-Bromo-4-chloro-3-indolyl β-D-cellobioside is a chromogenic compound used to detect cellobiohydrolases.

Mca-YVADAP-Lys(Dnp)-OH is a fluorogenic substrate for caspase-1 and angiotensin-converting enzyme 2 (ACE2).

Gly-Gly-AMC is a fluorogenic peptide substrate. Gly-Gly-AMC can be used to assess bacterial protease activity of P. aeruginosa and S. aureus.

Mca-VDQMDGWK-(Dnp)-NH2 is a fluorogenic substrate for caspase-3 and can be used to quantify caspase-3 activity.

Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 is a fluorogenic substrate for matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-26. Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 can be used to quantify MMP-1, MMP-3 and MMP-26 activity.

Mca-DEVDAP-K(Dnp)-OH is a fluorogenic substrate for caspase-3.

Purple-β-D-Gal is a chromogenic β-galactosidase substrate. Intracellular enzymatic hydrolysis of Purple-β-D-Gal generates free indoxyl molecules, which undergo in situ oxidation and subsequent dimerization to produce chromogenic, water-insoluble, indigo precipitates. Purple-β-D-Gal can be used for the detection of β-galactosidase activity.

5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm).

Thymolphthalein monophosphate disodium hydrate is a chromogenic substrate for the determination of acid phosphatase and alkaline phosphatase. Thymolphthalein is released during the reaction, increases the pH of the medium for easy detection, produces color and stops hydrolysis. Thymolphthalein monophosphate disodium hydrate can be used for the specific detection of prostatic phosphatase in serum.

Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity.

7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A₂ (cPLA₂) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA₂.

C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid. C12FDGlcU can be useful for the detection of β-glucuronidase (GUS) gene expression. C12FDGlcU can enter the cells and then be cleaved by β-glucuronidase, generating the yellow-colored, green-fluorescent fluorescein (Abs/Em of the reaction product: 495/518 nm).

2-Methoxy-2,4-diphenylfuran-3-one is a fluorescent compound which can be used to label gelatin as a substrate for detection of the gelatin degrading MMP-2 and MMP-9 by zymography.

Z-Arg-Arg-4MβNA triacetate is a cathepsin B-specific substrate and can produce fluorescent end product 4MβNA (λ_ex = 355 nm, λ_em = 430 nm).

4-Methylumbelliferyl phosphate (2-amino-2-methyl-1,3-propanediol) is a fluorescent substrate, can be used as substrate buffer of enzyme assay.

H-Ala-4MβNA hydrochloride is a specific fluorogenic substrate for aminopeptidase M.

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) is a chromogenic substrate to peroxidase enzymes. Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone can form a purple indamine dye with peroxidase enzymes and peroxides.