Ac-DNLD-AMC
Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity.
For research use only. We do not sell to patients.
- CAS No.: 958001-92-8
- Formula: C30H38N6O12
- Molecular Weight:674.66
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of caspase[1]:
1.Cells were harvested and washed with PBS.
2.After centrifugation, they were resuspended in PBS at a concentration of 2 × 106 cells/100 μL.
3.25 μL of the suspension was added to a 96-well plate and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer (100 mM HEPES, 10 % sucrose, 5 mM DTT, 0.001 % NP-40, and 0.1 % CHAPS, pH 7.2).
4.Ac-DNLD-AMC was monitored by AMC liberation in a multimode detector using 380-nm excitation and 460-nm emission wavelengths.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
445
360
Chemical Information
-
CAS No. 958001-92-8
-
Molecular Weight 674.66
-
Formula C30H38N6O12
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Kulikov AV, et al. Targeting mitochondria by α-tocopheryl succinate overcomes hypoxia-mediated tumor cell resistance to treatment. Cell Mol Life Sci. 2014 Jun;71(12):2325-33. [Content Brief]
[2]. Yoshida K, et al. Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells. Cell Immunol. 2009;255(1-2):61-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz