7-Hydroxycoumarinyl-γ-linolenate
7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2.
For research use only. We do not sell to patients.
- CAS No.: 161180-12-7
- Formula: C27H34O4
- Molecular Weight:422.56
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
cPLA2α activity assay[1][2]:
1. Sonicate 200 μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960 μM Triton X-100 (HY-Y1883A), 60 μM DTPC, 50 μM 7-hydroxycoumarinyl-γ-linolenate, 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH = 7.2), 0.3 mM EDTA (HY-Y0682), 1 mM CaCl2 and 300 mM KCl.
2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360 nm and emission at 460 nm.
3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
460
Excitation (Ex)
380
Chemical Information
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CAS No. 161180-12-7
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Molecular Weight 422.56
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Formula C27H34O4
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SMILES
CCCCC/C=C\C/C=C\C/C=C\CCCCC(OC1=CC=C(C(O2)=C1)C=CC2=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Xiao Q, et al. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep. 2016 Apr 29;6:25298. [Content Brief]
[2]. Huang Z, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
7-Hydroxycoumarinyl-γ-linolenate Related Classifications
HY-D1647 Related Classifications
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