7-Hydroxycoumarinyl arachidonate
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7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer.
For research use only. We do not sell to patients.
- Purity: 99.19%
- CAS No.: 161180-11-6
- Formula: C29H36O4
- Molecular Weight:448.59
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Storage:
Solution, -20°C, 2 years
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 161180-11-6
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Appearance Liquid
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Molecular Weight 448.59
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Formula C29H36O4
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Color Colorless to light yellow
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SMILES
CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(OC1=CC=C2C=CC(OC2=C1)=O)=O
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Synonyms
7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Purity & Documentation
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Data Sheet (261 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Yuren Wang, et al. A fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening. Assay Drug Dev Technol. 2008 Jun;6(3):387-93. [Content Brief]
[2]. Z Huang, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5. [Content Brief]
[3]. R T Pickard, et al. Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine. J Biol Chem. 1996 Aug 9;271(32):19225-31. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
7-Hydroxycoumarinyl arachidonate Related Classifications
HY-116141 Related Classifications
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