2. Metabolic Enzyme/Protease
  3. Phospholipase MAGL
  4. 7-Hydroxycoumarinyl arachidonate

7-Hydroxycoumarinyl arachidonate  (Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)

Cat. No.: HY-116141 Purity: ≥98.0%
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7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer.

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7-Hydroxycoumarinyl arachidonate Chemical Structure

7-Hydroxycoumarinyl arachidonate Chemical Structure

CAS No. : 161180-11-6

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5 mg (55.73 mM * 200 μl ethanol) USD 150 In-stock
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7-Hydroxycoumarinyl arachidonate Related Antibodies

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Description

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3].

In Vitro

MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Hydroxycoumarinyl arachidonate Related Antibodies
Molecular Weight

448.59

Formula

C29H36O4
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(OC1=CC=C2C=CC(OC2=C1)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years
Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (222.92 mM; Need ultrasonic and warming)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

COA (238 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.2292 mL 11.1460 mL 22.2921 mL 55.7302 mL
5 mM 0.4458 mL 2.2292 mL 4.4584 mL 11.1460 mL
10 mM 0.2229 mL 1.1146 mL 2.2292 mL 5.5730 mL
15 mM 0.1486 mL 0.7431 mL 1.4861 mL 3.7153 mL
20 mM 0.1115 mL 0.5573 mL 1.1146 mL 2.7865 mL
25 mM 0.0892 mL 0.4458 mL 0.8917 mL 2.2292 mL
30 mM 0.0743 mL 0.3715 mL 0.7431 mL 1.8577 mL
40 mM 0.0557 mL 0.2787 mL 0.5573 mL 1.3933 mL
50 mM 0.0446 mL 0.2229 mL 0.4458 mL 1.1146 mL
60 mM 0.0372 mL 0.1858 mL 0.3715 mL 0.9288 mL
80 mM 0.0279 mL 0.1393 mL 0.2787 mL 0.6966 mL
100 mM 0.0223 mL 0.1115 mL 0.2229 mL 0.5573 mL
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7-Hydroxycoumarinyl arachidonate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Hydroxycoumarinyl arachidonate161180-11-67-HCA Umbelliferyl Arachidonate 7-HC-arachidonatePhospholipaseMAGLMonoacylglycerol lipasePLA2Arachidonicacid7-hydroxylcoumarin7-HCfluorometerInhibitorinhibitorinhibit

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