Endo-β-N-acetylglucosaminidase D
Based on 1 Customer Validation
Endo-β-N-acetylglucosaminidase D (Endo D), isolated from Streptococcus pneumoniae. Endo-β-N-acetylglucosaminidase D hydrolyzes Fc N-glycan of intact IgG antibodies after sequential removal of the sialic acid, galactose, and internal GlcNAc residues in the N-glycan. Endo-β-N-acetylglucosaminidase D possesses transglycosylation activity with sugar oxazoline as the donor substrate.
For research use only. We do not sell to patients.
- CAS No.: 37278-88-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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HY-E70131
Endo H, Streptomyces picatus -
HY-E70069
Endo S2, Streptococcus pyogenes -
HY-E70134
Endo-β-N-acetylglucosaminidase F3 -
HY-E70135
Endo-β-N-acetylglucosaminidase F1 -
HY-E70133
Endo-β-N-acetylglucosaminidase F2 -
HY-E70068
Endo-β-N-acetylglucosaminidase (Endo S) -
HY-E70070
Endo-β-N-acetylglucosaminidase (Endo A) -
HY-E70069GL
Endo S2, Streptococcus pyogenes (GMP Like) -
HY-P2999
Endo-β-N-acetylglucosaminidase -
HY-B2220
Cellulase -
HY-126386
Pectinase, aspergillus niger -
HY-B2193
α-Amylase
Biological Activity
Description
IC50 & Target
others
In Vitro
Protocol
1) For 10 μg of Fetuin, add 1 μL of 500 mM sodium acetate (pH 7.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL Endo D, gently pipet to mix;
3) Incubate at 37 °C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry.
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.2.1.96
Specific Activity
≥1000 U/mL
Unit Definition
One unit is defined as the amount of enzyme that split 10 μg of denatured Fetuin > 95% of the carbohydrate in 1 hour at 37°C in 10 μL total reaction volume.
Chemical Information
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CAS No. 37278-88-9
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Endo-b-N-acetylglucosaminidase D]
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Synonyms
Endo D
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Endo-β-N-acetylglucosaminidase D Related Classifications
HY-E70132 Related Classifications
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