Endo-β-N-acetylglucosaminidase F3  (Synonyms: Endo F3)

Endo-β-N-acetylglucosaminidase D (Endo F3) cleaves free or Asparagine-linked triantennary oligosaccharides or α1-6 fucosylated biantennary oligosaccharides, as well as triamnnosyl chitobiose core structures^[1].

others

Protocol
1) For 10 μg of IgG, add 1 μL of 500 mM sodium acetate (pH 7.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL Endo F3, pipe gently to mix;
3) Incubate at 37 °C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry.
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

Liquid

Colorless to light yellow

[Endo-b-N-acetylglucosaminidase F3]

3.2.1.96

≥100 U/mL

One unit is defined as the amount of enzyme that split 10 μg of human IgG > 95% of the carbohydrate in 1 hour at 37°C in 10 μL total reaction volume.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. R B Trimble, et al. Identification of distinct endoglycosidase (endo) activities in Flavobacterium meningosepticum: endo F1, endo F2, and endo F3. Endo F1 and endo H hydrolyze only high mannose and hybrid glycans. J Biol Chem. 1991 Jan 25;266(3):1646-51.  [Content Brief]