Endo H, Streptomyces picatus
Based on 2 publication(s) in Google Scholar
Endo H, Streptomyces picatus (Endo-β-N-acetylglucosaminidase H), isolated from Streptomyces plicatus, hydrolyzes the central glycosidic bond of the β1, 4-di-N-acetylchitobiose core in asparagine-linked oligosaccharides.
For research use only. We do not sell to patients.
- CAS No.: 37278-88-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Endo H, Streptomyces picatus
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Biological Activity
Description
IC50 & Target
others
In Vitro
Protocol
1) For 10 μg of RNase B, add 1 μL of 500 mM sodium acetate (pH 7.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of EndoH, pipe gently to mix;
3) Incubate at 37°C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry.
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
EC Number
3.2.1.96
Specific Activity
≥5000 U/mg protein
Unit Definition
One unit is defined as the amount of enzyme that split 10 μg of human RNase B > 95% of the carbohydrate in 1 hour at 37°C in 10 μL total reaction volume.
Technical Parameters
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Source
E. coli
Chemical Information
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CAS No. 37278-88-9
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Endo H, Streptomyces picatus]
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Synonyms
Endo-β-N-acetylglucosaminidase H
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Endo H, Streptomyces picatus Related Classifications
HY-E70131 Related Classifications
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