Endo-β-N-acetylglucosaminidase F1  (Synonyms: Endo F1)

Endo-β-N-acetylglucosaminidase F1 (Endo F1) cleaves Asparagine-linked high mannose and some hybrid oligosaccharides^[1].

others

Protocol
1) Dissolve 1-20 μg of glycoprotein in deionized water, add 1 μL of 500 mM sodium acetate solution (pH5.0), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of Endo F1, pipe gently to mix;
3) Incubate at 37 °C for 60 min;
4) For SDS-PAGE analysis or HPLC analysis.
Note: Most common biological buffers are also suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

Liquid

Colorless to light yellow

[Endo-b-N-acetylglucosaminidase F1]

3.2.1.96

≥300 U/mL

One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from denatured 5 μg RNase B at 37°C, reacting 1 hour, in a 10 μL reaction system.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Jie Ao, et al. The N-Linked Outer Chain Mannans and the Dfg5p and Dcw1p Endo-α-1,6-Mannanases Are Needed for Incorporation of Candida albicans Glycoproteins into the Cell Wall. Eukaryot Cell. 2015 Aug;14(8):792-803.  [Content Brief]