Endo-β-N-acetylglucosaminidase F1
Based on 1 Customer Validation
Endo-β-N-acetylglucosaminidase F1 (Endo F1) cleaves Asparagine-linked high mannose and some hybrid oligosaccharides.
For research use only. We do not sell to patients.
- CAS No.: 37278-88-9
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
-
HY-E70131
Endo H, Streptomyces picatusEndoglycosidases -
HY-E70069
Endo S2, Streptococcus pyogenesCatalyzing Enzyme -
HY-E70134
Endo-β-N-acetylglucosaminidase F3Endoglycosidases -
HY-E70133
Endo-β-N-acetylglucosaminidase F2Endoglycosidases -
HY-E70068
Endo-β-N-acetylglucosaminidase (Endo S)Hydrolase -
HY-E70132
Endo-β-N-acetylglucosaminidase DEndoglycosidases -
HY-E70070
Endo-β-N-acetylglucosaminidase (Endo A)Catalyzing Enzyme -
HY-E70069GL
Endo S2, Streptococcus pyogenes (GMP Like)Biochemical Assay Reagent -
HY-P2999
Endo-β-N-acetylglucosaminidaseDeglycosylating Enzyme -
HY-B2220
CellulaseEnzyme -
HY-126386
Pectinase, aspergillus nigerEnzyme -
HY-B2193
α-AmylaseHydrolase Enzyme
Biological Activity
Description
IC50 & Target
others
In Vitro
Protocol
1) Dissolve 1-20 μg of glycoprotein in deionized water, add 1 μL of 500 mM sodium acetate solution (pH5.0), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of Endo F1, pipe gently to mix;
3) Incubate at 37 °C for 60 min;
4) For SDS-PAGE analysis or HPLC analysis.
Note: Most common biological buffers are also suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.
| Specification | Type | Components | Volume | Quantity |
| 1 KU | A | Endo-β-N-acetylglucosaminidase F1 | 1 KU | 1 |
| 1 KU | B | 10× Glycoprotein Denaturing Buffer | 1 mL | 1 |
| 1 KU | C | 10× GlycoBuffer | 1 mL | 1 |
| 1 KU | D | 10% NP-40 | 1 mL | 1 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
EC Number
3.2.1.96
Specific Activity
≥300 U/mL
Unit Definition
One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from denatured 5 μg RNase B at 37°C, reacting 1 hour, in a 10 μL reaction system.
Chemical Information
-
CAS No. 37278-88-9
-
Appearance Liquid
-
Color Colorless to light yellow
-
SMILES
[Endo-b-N-acetylglucosaminidase F1]
-
Synonyms
Endo F1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (269 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Endo-β-N-acetylglucosaminidase F1 Related Classifications
HY-E70135 Related Classifications
Powered by Bioz