Endo-β-N-acetylglucosaminidase (Endo S)
Based on 1 Customer Validation
Endo-β-N-acetylglucosaminidase (Endo S) is a family 18 glycosyl hydrolase secreted by Streptococcus pyogenes. Endo-β-N-acetylglucosaminidase (Endo S) exclusively hydrolyzes the β-1,4-di-N-acetylchitobiose core of the asparagine-linked complex-type glycan on Asn-297 of the γ-chains of IgG antibody.
For research use only. We do not sell to patients.
- CAS No.: 37278-88-9
- Molecular Weight:136 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
-
HY-E70069
Endo S2, Streptococcus pyogenes -
HY-E70131
Endo H, Streptomyces picatus -
HY-E70134
Endo-β-N-acetylglucosaminidase F3 -
HY-E70135
Endo-β-N-acetylglucosaminidase F1 -
HY-E70133
Endo-β-N-acetylglucosaminidase F2 -
HY-E70132
Endo-β-N-acetylglucosaminidase D -
HY-E70070
Endo-β-N-acetylglucosaminidase (Endo A) -
HY-E70069GL
Endo S2, Streptococcus pyogenes (GMP Like) -
HY-P2999
Endo-β-N-acetylglucosaminidase -
HY-B2220
Cellulase -
HY-126386
Pectinase, aspergillus niger -
HY-B2193
α-Amylase
Biological Activity
Description
In Vitro
Product Information
This product is expressed by recombinant E. coli, with N-terminal His-tag, molecular weight of 136 kDa, and buffer components of 20 mM Tris-HCl (pH7.5), 200 mM NaCl, and 10% glycerol. It can be used for antibody glycosylation modification and analysis.
Instructions
1. Add 1 μL of 500 mM sodium phosphate (pH4.5) to 10 μg bovine fibrinogen, then add Endo S and incubate at 37°C for 1 hour.
2. Analyze by SDS-PAGE or mass spectrometry.
Notes
The incubation time or enzyme amount needs to be optimized according to the actual situation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Unit Definition
One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from 5 μg human IgG at 37°C, reacting 1 hour, in a 10 μL reaction system.
Technical Parameters
-
Source
E. coli
-
Tag
N-terminal His-tag
Chemical Information
-
CAS No. 37278-88-9
-
Appearance Liquid
-
Molecular Weight 136 kDa
-
Color Colorless to light yellow
-
SMILES
[Endo-b-N-acetylglucosaminidase(Endo S)]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (265 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Endo-β-N-acetylglucosaminidase (Endo S) Related Classifications
HY-E70068 Related Classifications
Powered by Bioz