Endo-β-N-acetylglucosaminidase F2
Based on 1 Customer Validation
Endo-β-N-acetylglucosaminidase F2 (Endo F2), a highly specific endoglycosidase, cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. Endo F2 is not active on hybrid or tri- and tetra-antennary oligosaccharides.
For research use only. We do not sell to patients.
- CAS No.: 37278-88-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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HY-E70131
Endo H, Streptomyces picatus -
HY-E70069
Endo S2, Streptococcus pyogenes -
HY-E70134
Endo-β-N-acetylglucosaminidase F3 -
HY-E70135
Endo-β-N-acetylglucosaminidase F1 -
HY-E70068
Endo-β-N-acetylglucosaminidase (Endo S) -
HY-E70132
Endo-β-N-acetylglucosaminidase D -
HY-E70070
Endo-β-N-acetylglucosaminidase (Endo A) -
HY-E70069GL
Endo S2, Streptococcus pyogenes (GMP Like) -
HY-P2999
Endo-β-N-acetylglucosaminidase -
HY-B2220
Cellulase -
HY-126386
Pectinase, aspergillus niger -
HY-B2193
α-Amylase
Biological Activity
Description
IC50 & Target
others
In Vitro
Protocol
1) For 10 μg of glycoprotein, add 1 μl of 500 mM sodium acetate (pH 5.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of Endo F2, pipe gently to mix;
3) Incubate at 37 °C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry;
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.
| Specification | Type | Components | Volume | Quantity |
| 400 U | A | Endo-β-N-acetylglucosaminidase F2 | 400 U | 1 |
| 400 U | B | 500 mM Sodium acetate | 1 mL | 1 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.2.1.96
Specific Activity
≥100 U/mL
Unit Definition
One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from denatured 10 μg Bovine blood fibrinogen at 37°C, reacting 1 hour, in a 10 μL reaction system.
Chemical Information
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CAS No. 37278-88-9
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Endo-b-N-acetylglucosaminidase F2]
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Synonyms
Endo F2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Endo-β-N-acetylglucosaminidase F2 Related Classifications
HY-E70133 Related Classifications
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