FAK-IN-6
FAK-IN-6 is a potent FAK inhibitor with an IC50 value of 1.415 nM. FAK-IN-6 has anti-proliferative activity against certain cancer cell lines. FAK-IN-6 can be used for researching pancreatic cancer.
For research use only. We do not sell to patients.
- CAS No.: 3033299-38-3
- Formula: C25H31ClN5O6PS
- Molecular Weight:596.04
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
IC50: 1.415 nM (FAK)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| ASPC1 | IC50 |
0.1596 μM
Compound: 9h
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Antiproliferative activity against human ASPC1 cells harboring KRAS,TP53,CDKN2A and DPC4/SMAD4 mutations assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Antiproliferative activity against human ASPC1 cells harboring KRAS,TP53,CDKN2A and DPC4/SMAD4 mutations assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
| KM3/BTZ | IC50 |
2.286 μM
Compound: 9h
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Antiproliferative activity against bortezomib- resistant human KM3/BTZ cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Antiproliferative activity against bortezomib- resistant human KM3/BTZ cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
| L02 | IC50 |
1.398 μM
Compound: 9h
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Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
| MIA PaCa-2 | IC50 |
0.7679 μM
Compound: 9h
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Antiproliferative activity against human MIA PaCa-2 cells harboring KRAS,TP53,CDKN2A and DPC4/SMAD4 mutations assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Antiproliferative activity against human MIA PaCa-2 cells harboring KRAS,TP53,CDKN2A and DPC4/SMAD4 mutations assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
| NCI-H1975 | IC50 |
1.355 μM
Compound: 9h
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Antiproliferative activity against gefitinib-resistant human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Antiproliferative activity against gefitinib-resistant human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
| Raji | IC50 |
1.13 μM
Compound: 9h
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Antiproliferative activity against human Raji cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
Antiproliferative activity against human Raji cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay
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[PMID: 35872546] |
In Vitro
FAK-IN-6 (compound 9h) (0-10 μM; 72 h) has anti-proliferative activity against pancreatic cancer cells, lung cancer cells and lymphoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:AsPC-1, PaCa-2, H1975, KM3/BTZ, Raji and L-02[1]
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Concentration:0-10 μM
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Incubation Time:72 h
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Result:Exhibited anti-proliferative activity against AsPC-1, PaCa-2, H1975, KM3/BTZ, Raji and L-02 with IC50s of 0.9886 ± 0.0086 μM, 5.274 ± 0.9312 μM, 2.918 ± 0.0821 μM, 2.315 ± 0.2969 μM, 1.320 ± 0.2973 μM and 1.220 ± 0.2683 μM.
Chemical Information
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CAS No. 3033299-38-3
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Molecular Weight 596.04
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Formula C25H31ClN5O6PS
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SMILES
O=C(NC1=CC=C(NC2=NC=C(Cl)C(NC3=CC=CC=C3S(=O)(C(C)C)=O)=N2)C=C1)CP(OCC)(OCC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FAK-IN-6 Related Classifications
HY-150730 Related Classifications
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