2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Liposome
  4. Fluorescent DOTAP

Fluorescent DOTAP  (Synonyms: NBD-DOTAP)

製品番号: HY-143702 純度: 95.95%
COA 取扱説明書 Technical Support

Fluorescent DOTAP (NBD-DOTAP) is a cationic lipid which can be used for nucleic acid and protein delivery. Fluorescent DOTAP is labeled with a fluorophore NBD (maximum excitation/emission wavelength ∼463/536 nm). Fluorescent DOTAP can be used for gene delivery systems, drug delivery, as well as cell imaging and nanocarrier tracking. Fluorescent DOTAP is an ideal candidate for both biological and pharmaceutical formulation (e.g. co-delivery of vaccines) research.

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Fluorescent DOTAP

Fluorescent DOTAP 構造式

CAS 番号 : 1010076-97-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 625 在庫あり
Solution
10 mM * 1 mL in DMSO USD 625 在庫あり
Solid
1 mg $140 在庫あり
5 mg $400 在庫あり
10 mg $680 在庫あり
25 mg $1350 在庫あり
50 mg $2200 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 1 publication(s) in Google Scholar

Fluorescent DOTAP 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Fluorescent DOTAP (NBD-DOTAP) is a cationic lipid which can be used for nucleic acid and protein delivery. Fluorescent DOTAP is labeled with a fluorophore NBD (maximum excitation/emission wavelength ∼463/536 nm). Fluorescent DOTAP can be used for gene delivery systems, drug delivery, as well as cell imaging and nanocarrier tracking. Fluorescent DOTAP is an ideal candidate for both biological and pharmaceutical formulation (e.g. co-delivery of vaccines) research[1][3].

体外実験

Fluorescent DOTAP results in a higher hemolysis with increasing concentration of DOTAP in fusogenic liposomes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescent DOTAP 関連抗体
体内実験

Fluorescent DOTAP (0.5% (w/w), s.c., one single dose) increases the numbers of CD11c+ cells among total lymph node cells more than 2.5-fold compared to the untreated at 4 h in C57BL/6 female mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 female mice (6-7 weeks old) with subcutaneous tumors[3]
Dosage: 0.5% (w/w)
Administration: Subcutaneous injection (s.c.), one single dose
Result: Showed neither CD8+ or CD8- cells took up NBD after fluorescent DOTAP injection.
Demonstrated the main uptake is by DC (80%) soon after immunization.
Induced migration of activated DC to the DLN.
Resulted in DC-T cell interactions and eliciting T cell responses.
分子量

710.34

分子式

C36H60ClN5O7
CAS 番号
Appearance

Solid

Color

Orange to reddish brown

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C21)=O)C[N+](C)(C)C)=O.[Cl-]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 20 mg/mL (28.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4078 mL 7.0389 mL 14.0778 mL
5 mM 0.2816 mL 1.4078 mL 2.8156 mL
10 mM 0.1408 mL 0.7039 mL 1.4078 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (2.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 95.95%

COA (240 KB) HNMR (260 KB) RP-HPLC (262 KB) LCMS (231 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4078 mL 7.0389 mL 14.0778 mL 35.1944 mL
5 mM 0.2816 mL 1.4078 mL 2.8156 mL 7.0389 mL
10 mM 0.1408 mL 0.7039 mL 1.4078 mL 3.5194 mL
15 mM 0.0939 mL 0.4693 mL 0.9385 mL 2.3463 mL
20 mM 0.0704 mL 0.3519 mL 0.7039 mL 1.7597 mL
25 mM 0.0563 mL 0.2816 mL 0.5631 mL 1.4078 mL
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Fluorescent DOTAP Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescent DOTAP1010076-97-7NBD-DOTAPFluorescent DyeLiposomecationic lipidnucleic acidprotein deliveryInhibitorinhibitorinhibit

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製品名:
Fluorescent DOTAP
製品番号:
HY-143702
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