Cy7-NH2 chloride

Guide
(The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.1 Solvent: Not specified in the provided literature; section omitted.
1.2 Concentration recommendation: Not specified in the provided literature; section omitted.

2. Working Solution Preparation
2.1 Diluent: Not specified in the provided literature; section omitted.
2.2 Working concentration: Not specified in the provided literature; section omitted.
2.3 Note: Adjust working solution concentration as needed; prepare fresh before use.

3. Staining Procedure
3.1 Sample type: Mouse liver tissue samples.[1]
3.2 Incubation conditions: Administer Cy7-NH₂ via daily oral gavage for 2 weeks to CCl₄-induced fibrotic mice; Cy7-NH₂ emits magenta fluorescence.
3.3 Washing steps: Not specified in the provided literature; section omitted.

4. Controls
4.1 Cy7-NH₂ is used as a control for Cy7-IPA to verify target cell specificity of IPA.

5. Detection & Analysis
5.1 Instrument: Multiplex immunohistochemical (mIHC) imaging system or fluorescence microscopy.
5.1.1 Ex/Em wavelength: Not specified in the provided literature; section omitted.
5.2 Result analysis:
5.2.1 Fluorescence localization: Magenta Cy7-NH₂ signals do not predominantly accumulate in F4/80⁺ macrophages, distinguishing it from Cy7-IPA which targets these cells.
5.2.2 Color changes: Cy7-NH₂ produces magenta fluorescence; co-staining includes blue DAPI (nuclei), green F4/80 (macrophages), yellow α-SMA (hepatic stellate cells), and gray HNF4α (hepatocytes).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.