CJJ300
Based on 1 Customer Validation
CJJ300 is a transforming growth factor-β (TGF-β) inhibitor with an IC50 of 5.3 µM. CJJ300 inhibits TGF-β signaling by disrupting the formation of the TGF-β-TβR-I-TβR-II signaling complex.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.36%
- No. CAS: 1807631-83-9
- Fòrmula: C30H33N3
- Peso molecular:435.60
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
IC50 & Target
IC50: 5.3 µM (TGF-β1-induced luciferase)[1]
In Vitro
CJJ300 disturbs protein-protein interactions and prevents TGF-β receptor dimerization (IC50 = 23.6 ± 5.8 µM)[1].
CJJ300 (0-80 µM, 2 h) inhibits the phosphorylation of intracellular mediators in the downstream TGF-β signaling pathways, and suppresses the expression of markers of EMT (epithelial-mesenchymal transition) without cytotoxicity[1].
CJJ300 suppresses TGF-β induced cell migration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human A549 lung epithelial cells
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Concentration:20, 40, and 80 µM
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Incubation Time:2 h
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Result:Significantly attenuated the increasement of P-Smad2/Smad3, P-Erk1/2 and P-Akt induced by TGF-β. Down regulated the expression of EMT-associated proteins, including fibronectin, α-SMA and MMP-2 in a dose-dependent manner.
Chemical Information
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No. CAS 1807631-83-9
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Appearance Solid
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Peso molecular 435.60
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Fòrmula C30H33N3
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Color White to off-white
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SMILES
CC1=C(C(C)=C(C(C)=C1CNC2=CC=CC=C2)CNC3=CC=CC=C3)CNC4=CC=CC=C4
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (229.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2957 mL | 11.4784 mL | 22.9568 mL | 57.3921 mL |
| 5 mM | 0.4591 mL | 2.2957 mL | 4.5914 mL | 11.4784 mL | |
| 10 mM | 0.2296 mL | 1.1478 mL | 2.2957 mL | 5.7392 mL | |
| 15 mM | 0.1530 mL | 0.7652 mL | 1.5305 mL | 3.8261 mL | |
| 20 mM | 0.1148 mL | 0.5739 mL | 1.1478 mL | 2.8696 mL | |
| 25 mM | 0.0918 mL | 0.4591 mL | 0.9183 mL | 2.2957 mL | |
| 30 mM | 0.0765 mL | 0.3826 mL | 0.7652 mL | 1.9131 mL | |
| 40 mM | 0.0574 mL | 0.2870 mL | 0.5739 mL | 1.4348 mL | |
| 50 mM | 0.0459 mL | 0.2296 mL | 0.4591 mL | 1.1478 mL | |
| 60 mM | 0.0383 mL | 0.1913 mL | 0.3826 mL | 0.9565 mL | |
| 80 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7174 mL | |
| 100 mM | 0.0230 mL | 0.1148 mL | 0.2296 mL | 0.5739 mL |
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