D-Cysteic acid
D-Cysteic acid is a competitive inhibitor of the aspartate:alanine antiporter (AspT) from Tetragenococcus halophilus. For L-aspartate self-exchange, D-Cysteic acid has a Ki of 0.14 mM and an IC50 of 0.19 mM; for L-alanine self-exchange, it has a Ki of 5.0 mM and an IC50 of 9.0 mM. D-Cysteic acid occupies a distinct substrate-binding site within the substrate transport pathway of AspT, preventing the binding of natural substrates to the transporter. D-Cysteic acid can be used in studies related to substrate recognition and transport mechanisms of transporters.
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- No. CAS: 35554-98-4
- Fòrmula: C3H7NO5S
- Peso molecular:169.15
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
Actividad biológica
Descripciòn
In Vitro
D-cysteic acid competitively inhibits L-aspartate and L-alanine self-exchange via reconstituted Tetragenococcus halophilus AspT, with greater potency against L-aspartate self-exchange (Ki = 0.14 mM; IC50 = 0.19 mM) than L-alanine self-exchange (Ki = 5.0 mM; IC50=9.0 mM)[1].
D-Cysteic acid is formed via oxidation of L-cysteine with performic acid at 50°C for 15 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 35554-98-4
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Peso molecular 169.15
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Fòrmula C3H7NO5S
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SMILES
[C@@H](CS(=O)(=O)O)(C(O)=O)N
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)