HKSOX-1m (5/6-mixture) (hydrobromide)

Name: HKSOX-1m (5/6-mixture) (hydrobromide)
Brand: MedChemExpress
SKU: HY-D1156A
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1156A Purity: 97.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

HKSOX-1m (5/6-mixture) hydrobromide is a O₂ fluorescent probe for mitochondria-targeting (Ex/Em=509/534nm; green), exhibiting excellent selectivity and sensitivity toward O₂ over a broad range of pH, strong oxidants, and abundant reductants found in cells.

For research use only. We do not sell to patients.

HKSOX-1m (5/6-mixture) (hydrobromide) Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 375	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 990	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1628	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 3256	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 4884	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 7326	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of HKSOX-1m (5/6-mixture) (hydrobromide):

HKSOX-1m (5/6-mixture) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

HKSOX-1m (5/6-mixture) hydrobromide (10 μM) sensitively captures signals for basal and Antimycin A (1 μM)-stimulated mitochondrial O₂ in differentiated human THP-1 cells, at very low laser power output (1% intensity at Ex 514 nm on Zeiss LSM 510 Meta)^[1].

1. Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKSOX-1m (5/6-mixture) hydrobromide in 79 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKSOX-1m (5/6-mixture) hydrobromide working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1258.72

Formula

C₄₉H₃₇Br₂F₁₀N₂O₁₀PS₂

Appearance

Solid

Emission (Em)

534

Excitation (Ex)

509

SMILES

O=C1OC(C(C2=C3F)=CC(F)=C3O[S](C(F)(F)F)(=O)=O)(C4=CC(C(N(CC5)CCN5CCCC[P+](C6=CC=CC=C6)(C7=CC=CC=C7)C8=CC=CC=C8)=O)=CC=C41)C(C(O2)=C9F)=CC(F)=C9O[S](C(F)(F)F)(=O)=O.O=C%10OC(C(C%11=C%12F)=CC(F)=C%12O[S](C(F)(F)F)(=O)=O)(C(C%10=C%13)=CC=C%13C(N(CC%14)CCN%14CCCC[P+](C%15=CC=CC=C%15)(C%16=CC=CC=C%16)C%17=CC=CC=C%17)=O)C(C(O%11)=C%18F)=CC(F)=C%18O[S](C(F)(F)F)(=O)=O.[Br-].Br.[Br-].Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (158.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7945 mL	3.9723 mL	7.9446 mL
5 mM	0.1589 mL	0.7945 mL	1.5889 mL
10 mM	0.0794 mL	0.3972 mL	0.7945 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (266 KB) HNMR (174 KB) RP-HPLC (195 KB)

Handling Instructions (2659 KB)

References

[1]. Jun Jacob Hu, et al. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc . 2015 Jun 3;137(21):6837-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7945 mL	3.9723 mL	7.9446 mL	19.8614 mL
	5 mM	0.1589 mL	0.7945 mL	1.5889 mL	3.9723 mL
	10 mM	0.0794 mL	0.3972 mL	0.7945 mL	1.9861 mL
	15 mM	0.0530 mL	0.2648 mL	0.5296 mL	1.3241 mL
	20 mM	0.0397 mL	0.1986 mL	0.3972 mL	0.9931 mL
	25 mM	0.0318 mL	0.1589 mL	0.3178 mL	0.7945 mL
	30 mM	0.0265 mL	0.1324 mL	0.2648 mL	0.6620 mL
	40 mM	0.0199 mL	0.0993 mL	0.1986 mL	0.4965 mL
	50 mM	0.0159 mL	0.0794 mL	0.1589 mL	0.3972 mL
	60 mM	0.0132 mL	0.0662 mL	0.1324 mL	0.3310 mL
	80 mM	0.0099 mL	0.0497 mL	0.0993 mL	0.2483 mL
	100 mM	0.0079 mL	0.0397 mL	0.0794 mL	0.1986 mL