Indocyanine green (solution) 

Indocyanine green (Foxgreen) (solution) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography (Ex/Em = 785/813 nm) ^[1].
Solvent and concentration: DMSO: 10 mM

At a concentration of 1000 μg/mL, Indocyanine green (Foxgreen) - photodynamic therapy (ICG-PDT) significantly induced the expression of BAX in HGF cells; however, laser irradiation and ICG had no significant effect on the expression of the BCL-2 gene [1].

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of Working Solution^[3] Dilute to 0.03 - 10 μM using Tris buffer or other buffers (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and stored in a dark place.

2. Organizing the Staining 2.1 The experiment utilized New Zealand white rabbits with Langendorff perfusion for the heart.
2.2 Add Blebbistatin (20 μM) (HY-13813) and Biacetyl monoxime (5 mM) (HY-Y0413) to the perfusion solution to inhibit cardiac contraction while maintaining the electrical activity.
2.3 Gradually increase the concentration of Indocyanine green dye (from 0.03 μM to 10 μM) and add it to the perfusion solution to stain the heart.
2.4 The duration of the light signal recorded by the spectrometer is 3 seconds.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Description^[2].
Indocyanine green (ICG) can absorb the near IR laser energy and release heat in the dyed tissue. Pulsed laser treatment combined with Indocyanine green causes targeted tumor cell death in neuroblastoma tumor without capsule.
Indocyanine green injections and application of laser.
After tumor volume reached 1000 mm³, mice are anesthetized underwent laparotomy to expose the orthotopic neuroblastoma tumor, liver, and kidney; skin over the proximal leg is removed to expose the quadriceps.
Exposed tumor and organs are treated at multiple locations with a pulse or continuous wave laser (805 nm), or laser application after an injection of 10 μL of 0.857 mg/mL Indocyanine green (ICG).
Laser exposure is limited to a 3 mm diameter area for 20-120 seconds. Total energy delivered per treatment area is recorded.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

774.97

C₄₃H₄₇N₂NaO₆S₂

3599-32-4

O=S(CCCC[N+]1=C(/C=C/C=C/C=C/C=C2N(CCCCS(=O)(O[Na])=O)C3=C(C4=CC=CC=C4C=C3)C/2(C)C)C(C)(C)C5=C1C=CC6=CC=CC=C56)([O-])=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Gharesi S, et al. Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells. Photodiagnosis Photodyn Ther. 2017 Apr 21.  [Content Brief]

  [2]. Taylor JS, et al. Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction. J Pediatr Surg. 2018;53(12):2475-2479.  [Content Brief]

  [3]. Mačianskienė R, et al. Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Sci Rep. 2017;7(1):7983. Published 2017 Aug 11.  [Content Brief]