IRBP(651-670) human, mouse
Based on 1 Customer Validation
IRBP (651-670) human, mouse is an epitope and uveitis inducer naturally processed from the conserved region of native IRBP. IRBP (651-670) human, mouse increases the levels of proinflammatory cytokines in ocular tissues (IL-1β, IL-6, TNFα, IL-17A and IL-17F). IRBP (651-670) human, mouse is a IRBP fragment conserved among human, mouse and bovine species. IRBP (651-670) human, mouse induces experimental autoimmune uveitis. IRBP (651-670) human, mouse is applicable to research related to experimental autoimmune uveitis.
For research use only. We do not sell to patients.
- Purity: 99.13%
- CAS No.: 1977546-93-2
- Formula: C91H151N25O32
- Molecular Weight:2107.32
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Lipocalin Family Isoforms
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Biological Activity
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IL-1β |
IL-6 |
IL-17A |
IL-17F |
IRBP(651-670) human, mouse (0.2-20.0 μg/mL; 72 h) induces in vitro proliferation responses in draining lymph node cells from hIRBP651-670-immunized C57BL/6J mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J[1]
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Dosage:300 μg
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Administration:s.c.; single dose on day 0
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Result:Induced experimental autoimmune uveitis with a combined average histological score of 0.8 and 100% incidence reached by day 14 post-immunization.
Elevated proinflammatory cytokine levels in eye tissue extracts on day 14 post-immunization: IL-1β (400 pg/mL), IL-6 (6 pg/mL), TNFα (1.0 pg/mL), IL-17A (8 pg/mL), and IL-17F (8 pg/mL).
Caused more extensive retinal damage and higher inflammatory cell infiltration compared to hIRBP1-20 immunized mice.
Chemical Information
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CAS No. 1977546-93-2
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Appearance Solid
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Molecular Weight 2107.32
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Formula C91H151N25O32
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Color White to off-white
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Sequence
Leu-Ala-Gln-Gly-Ala-Tyr-Arg-Thr-Ala-Val-Asp-Leu-Glu-Ser-Leu-Ala-Ser-Gln-Leu-Thr
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Sequence Shortening
LAQGAYRTAVDLESLASQLT
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (47.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2.5 mg/mL (1.19 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 0.5 mg/mL (0.24 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (277 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Mattapallil MJ, et al. Characterization of a New Epitope of IRBP That Induces Moderate to Severe Uveoretinitis in Mice With H-2b Haplotype. Invest Ophthalmol Vis Sci. 2015 Aug;56(9):5439-49. [Content Brief]
[2]. Liu Y, et al. Galectin-3 regulates microglial activation and promotes inflammation through TLR4/MyD88/NF-kB in experimental autoimmune uveitis. Clin Immunol. 2022;236:108939. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.4745 mL | 2.3727 mL | 4.7454 mL | 11.8634 mL |
| DMSO | 5 mM | 0.0949 mL | 0.4745 mL | 0.9491 mL | 2.3727 mL |
| 10 mM | 0.0475 mL | 0.2373 mL | 0.4745 mL | 1.1863 mL | |
| 15 mM | 0.0316 mL | 0.1582 mL | 0.3164 mL | 0.7909 mL | |
| 20 mM | 0.0237 mL | 0.1186 mL | 0.2373 mL | 0.5932 mL | |
| 25 mM | 0.0190 mL | 0.0949 mL | 0.1898 mL | 0.4745 mL | |
| 30 mM | 0.0158 mL | 0.0791 mL | 0.1582 mL | 0.3954 mL | |
| 40 mM | 0.0119 mL | 0.0593 mL | 0.1186 mL | 0.2966 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
IRBP(651-670) human, mouse Related Classifications
HY-P1924 Related Classifications
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