IRBP(651-670) human, mouse

Name: IRBP(651-670) human, mouse
Brand: MedChemExpress
SKU: HY-P1924
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1924 Purity: 99.13%: Data Sheet
COA

SDS
Handling Instructions
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IRBP (651-670) human, mouse is an epitope and uveitis inducer naturally processed from the conserved region of native IRBP. IRBP (651-670) human, mouse increases the levels of proinflammatory cytokines in ocular tissues (IL-1β, IL-6, TNFα, IL-17A and IL-17F). IRBP (651-670) human, mouse is a IRBP fragment conserved among human, mouse and bovine species. IRBP (651-670) human, mouse induces experimental autoimmune uveitis. IRBP (651-670) human, mouse is applicable to research related to experimental autoimmune uveitis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

IRBP(651-670) human, mouse Chemical Structure

CAS No. : 1977546-93-2

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of IRBP(651-670) human, mouse:

IRBP(651-670) human, mouse TFA Get quote

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

IL-1β

IL-6

IL-17A

IL-17F

In Vitro

IRBP(651-670) human, mouse (0.2-20.0 μg/mL; 72 h) induces in vitro proliferation responses in draining lymph node cells from hIRBP651-670-immunized C57BL/6J mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

IRBP(651-670) human, mouse (300 μg; s.c.; single dose on day 0) induces experimental autoimmune uveitis in C57BL/6J mice with an average histological score of 0.8 and 100% incidence by day 14 post-immunization^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J^[1]
Dosage:	300 μg
Administration:	s.c.; single dose on day 0
Result:	Induced experimental autoimmune uveitis with a combined average histological score of 0.8 and 100% incidence reached by day 14 post-immunization. Elevated proinflammatory cytokine levels in eye tissue extracts on day 14 post-immunization: IL-1β (400 pg/mL), IL-6 (6 pg/mL), TNFα (1.0 pg/mL), IL-17A (8 pg/mL), and IL-17F (8 pg/mL). Caused more extensive retinal damage and higher inflammatory cell infiltration compared to hIRBP1-20 immunized mice.

Molecular Weight

2107.32

Formula

C₉₁H₁₅₁N₂₅O₃₂

CAS No.

1977546-93-2

Appearance

Solid

Color

White to off-white

Sequence

Leu-Ala-Gln-Gly-Ala-Tyr-Arg-Thr-Ala-Val-Asp-Leu-Glu-Ser-Leu-Ala-Ser-Gln-Leu-Thr

Sequence Shortening

LAQGAYRTAVDLESLASQLT

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (47.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.5 mg/mL (1.19 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4745 mL	2.3727 mL	4.7454 mL
5 mM	0.0949 mL	0.4745 mL	0.9491 mL
10 mM	0.0475 mL	0.2373 mL	0.4745 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 0.5 mg/mL (0.24 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (277 KB) SDS (254 KB)

COA (250 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Mattapallil MJ, et al. Characterization of a New Epitope of IRBP That Induces Moderate to Severe Uveoretinitis in Mice With H-2b Haplotype. Invest Ophthalmol Vis Sci. 2015 Aug;56(9):5439-49. [Content Brief]

[2]. Liu Y, et al. Galectin-3 regulates microglial activation and promotes inflammation through TLR4/MyD88/NF-kB in experimental autoimmune uveitis. Clin Immunol. 2022;236:108939. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.4745 mL	2.3727 mL	4.7454 mL	11.8634 mL
DMSO	5 mM	0.0949 mL	0.4745 mL	0.9491 mL	2.3727 mL
	10 mM	0.0475 mL	0.2373 mL	0.4745 mL	1.1863 mL
	15 mM	0.0316 mL	0.1582 mL	0.3164 mL	0.7909 mL
	20 mM	0.0237 mL	0.1186 mL	0.2373 mL	0.5932 mL
	25 mM	0.0190 mL	0.0949 mL	0.1898 mL	0.4745 mL
	30 mM	0.0158 mL	0.0791 mL	0.1582 mL	0.3954 mL
	40 mM	0.0119 mL	0.0593 mL	0.1186 mL	0.2966 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.