2. Metabolic Enzyme/Protease Immunology/Inflammation
  3. Lipocalin Family Interleukin Related
  4. IRBP(651-670) human, mouse TFA

IRBP (651-670) human, mouse TFA is an epitope and uveitis inducer naturally processed from the conserved region of native IRBP. IRBP (651-670) human, mouse TFA increases the levels of proinflammatory cytokines in ocular tissues (IL-1β, IL-6, TNFα, IL-17A and IL-17F). IRBP (651-670) human, mouse TFA is a IRBP fragment conserved among human, mouse and bovine species. IRBP (651-670) human, mouse TFA induces experimental autoimmune uveitis. IRBP (651-670) human, mouse TFA is applicable to research related to experimental autoimmune uveitis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

IRBP(651-670) human, mouse TFA

IRBP(651-670) human, mouse TFA Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of IRBP(651-670) human, mouse TFA:

Other Forms of IRBP(651-670) human, mouse TFA:

IRBP(651-670) human, mouse TFA Related Antibodies

Top Publications Citing Use of Products

View All Lipocalin Family Isoform Specific Products:

View All Isoforms
Retinol Binding Protein

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

IRBP (651-670) human, mouse TFA is an epitope and uveitis inducer naturally processed from the conserved region of native IRBP. IRBP (651-670) human, mouse TFA increases the levels of proinflammatory cytokines in ocular tissues (IL-1β, IL-6, TNFα, IL-17A and IL-17F). IRBP (651-670) human, mouse TFA is a IRBP fragment conserved among human, mouse and bovine species. IRBP (651-670) human, mouse TFA induces experimental autoimmune uveitis. IRBP (651-670) human, mouse TFA is applicable to research related to experimental autoimmune uveitis[1][2].

IC50 & Target[1][2]

IL-1β

 

IL-6

 

IL-17A

 

IL-17F

 

In Vitro

IRBP(651-670) human, mouse (0.2-20.0 μg/mL; 72 h) TFA induces in vitro proliferation responses in draining lymph node cells from hIRBP651-670-immunized C57BL/6J mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IRBP(651-670) human, mouse TFA Related Antibodies
In Vivo

IRBP(651-670) human, mouse (300 μg; s.c.; single dose on day 0) TFA induces experimental autoimmune uveitis in C57BL/6J mice with an average histological score of 0.8 and 100% incidence by day 14 post-immunization[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice[1]
Dosage: 300 μg
Administration: s.c.; single dose on day 0
Result: Induced experimental autoimmune uveitis with a combined average histological score of 0.8 and 100% incidence reached by day 14 post-immunization.
Elevated proinflammatory cytokine levels in eye tissue extracts on day 14 post-immunization: IL-1β (400 pg/mL), IL-6 (6 pg/mL), TNFα (1.0 pg/mL), IL-17A (8 pg/mL), and IL-17F (8 pg/mL).
Caused more extensive retinal damage and higher inflammatory cell infiltration compared to hIRBP1-20 immunized mice.
Molecular Weight

2107.32 (free acid)

Formula

C91H151N25O32.xC2HF3O2
Sequence

Leu-Ala-Gln-Gly-Ala-Tyr-Arg-Thr-Ala-Val-Asp-Leu-Glu-Ser-Leu-Ala-Ser-Gln-Leu-Thr
Sequence Shortening

LAQGAYRTAVDLESLASQLT
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

IRBP(651-670) human, mouse TFA Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IRBP(651-670) human, mouseLipocalin FamilyInterleukin RelatedILMHC tetramersepitope-specific T cellsT cell receptorC57BL/10J miceexperimental autoimmune uveoretinitisC57BL/6J micelymphoid organsI-Ab MHC class II moleculeH-2b haplotype miceuveitisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
IRBP(651-670) human, mouse TFA
Cat. No.:
HY-P1924A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.