Methylene blue indicator
Based on 1 publication(s) in Google Scholar
Methylene blue indicator is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.3%
- CAS 番号: 7220-79-3
- 分子式: C16H24ClN3O3S
- 分子量:373.90
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
MedChemExpress(MCE)の使用を引用している文献 Methylene blue indicator
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生物活性
Methylene blue indicator is widely employed as a redox indicator, exhibiting a blue color in its oxidized state and becoming colorless upon reduction[1].
Methylene blue indicator is commonly used for cell staining to visualize cell morphology, which primarily stains the nucleus due to its strong affinity for nucleic acids (DNA and RNA)[2].
Methylene blue indicator is also used for bacterial staining and capsule stainng[3].
Methylene blue indicator can be used as an indicator to recognize the single base mismatch in duplex DNA[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 7220-79-3
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性状 Solid
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分子量 373.90
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分子式 C16H24ClN3O3S
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Color Green to black
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SMILES
CN(C1=CC(SC2=C/C(C=CC2=N3)=[N+](C)\C)=C3C=C1)C.[3 H2O].[Cl-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Enhancing radiotherapy in triple-negative breast cancer with hesperetin-induced ferroptosis via AURKA targeting nanocomposites. [Abstract]2024 Nov 30;22(1):744. PMID: 39614277
溶剤 & 溶解度
DMSO : 6.67 mg/mL (17.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
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- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Farjami E, et al. DNA interactions with a Methylene Blue redox indicator depend on the DNA length and are sequence specific. Analyst. 2010 Jun;135(6):1443-8. [Content Brief]
[3]. Breakwell DP, et al. Differential staining of bacteria: capsule stain. Curr Protoc Microbiol. 2009 Nov;Appendix 3:Appendix 3I. [Content Brief]
[4]. Zhang Z, et al. Distinction of single base mismatches in duplex DNA using methylene blue as optical indicator. Analyst. 2010 Nov;135(11):2960-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6745 mL | 13.3726 mL | 26.7451 mL | 66.8628 mL |
| 5 mM | 0.5349 mL | 2.6745 mL | 5.3490 mL | 13.3726 mL | |
| 10 mM | 0.2675 mL | 1.3373 mL | 2.6745 mL | 6.6863 mL | |
| 15 mM | 0.1783 mL | 0.8915 mL | 1.7830 mL | 4.4575 mL |