FGF basic Protein, Salmon

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Based on 1 Customer Validation

FGF basic Protein, Salmon is the recombinant FGF-basic protein, expressed by E. coli, with tag free.

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  • Species: Others
  • Source: E. coli
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs

生物活性

製品説明

FGF basic Protein, Salmon is the recombinant FGF-basic protein, expressed by E. coli, with tag free.

Verified Bioactivity

Measure by its ability by a dose-response proliferation assay using murine Balb/c 3T3 cells. The ED50 for this effect is <1 ng/mL. The specific activity of this protein is > 1.0 × 106 IU/mg. (It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.)

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • アクセッション番号
  • 遺伝子ID
  • Molecular Construction
    • N-term
    • FGF basic (M1-R155)
      Accession # XP_035600424.1
    • C-term
  • Protein Length

    Full Length

  • 別名

    FGF2; BFGF; Prev. FGFB; Basic Fibroblast Growth Factor; Fibroblast Growth Factor 2 (Basic); Fibroblast Growth Factor; Heparin-Binding Growth Factor 2; Prostatropin; HBGF-2; FGF2A; FGF-2; FGF; Fibroblast Growth Factor 2; Basic Fibroblast Growth Factor BFGF

  • AA Sequence

    MATGEITTLPATPEDGGSGGFPPGNFKEPKRLYCKNGGYFLRINSNGSVDGIREKNDPHIKLQLQATSVGEVVIKGVSANRYLAMNGDGRLFGTRRTTDECYFMERLESNNYNTYRSRKYPDMYVALKRTGQHKSGSKTGPGQKAILFLPMSARR

  • 分子量

    Approximately 20 kDa, based on SDS-PAGE under non reducing (N) conditions.

  • 純度

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 7.8 mM Na2HPO4, 1.5 mM KH2PO4, 2.7 mM KCl, 500 mM NaCl.

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始) 濃度 (開始)
×
体積 (開始) 体積 (開始)
=
濃度 (終了) 濃度 (終了)
×
体積 (終了) 体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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