Frozen Section Embedding Medium

Guide (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
Method 1:
1. Remove the tissue block from the sucrose solution and immerse it in a 1:1 volume ratio mixture of 20 g/L sucrose and Frozen Section Embedding Medium embedding agent. Soak at room temperature for 2 hours.
2. Transfer the above tissue into Frozen Section Embedding Medium embedding medium and soak at room temperature for 4 hours; then replace with fresh Frozen Section Embedding Medium embedding medium and continue soaking at room temperature for 6 hours.
3. Place the specimen on the stage and section it using a cryostat, to a thickness of 10 μm. Mount the sections onto pretreated glass slides and store at -20°C.

Method Two:
1. Set the cryostat operating temperature to the range of -10°C to -25°C.
2. Apply a layer of embedding medium to the surface of the sample holder/disc, then gently press the tissue into the designated position on the sample holder/disc, and apply appropriate embedding medium around the tissue.

Note:
1. Tissue or embedding blocks may detach from the support due to insufficient embedding medium or misalignment of the tissue within the embedding medium.
2. Section tearing may be due to overfreezing of the tissue or damage or uncleanliness of the cutting edge.
3. Section thawing may be due to incorrect temperature of the cold chamber and the cutting edge.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.