Lysyl endopeptidase, Achromobacter sp
Based on 1 publication(s) in Google Scholar
Lysyl endopeptidase, Achromobacter sp (Lys-C) catalyzes carboxyl oxygen exchange reaction. Lysyl endopeptidase has higher substrate binding affinities and higher catalytic rates at the acidic pHs than at the alkaline pHs.
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- CAS No.: 123175-82-6
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Technical Parameters
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Chemical Information
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) Lysyl endopeptidase, Achromobacter sp
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Biological Activity
제품 설명
In Vitro
Product Information
Derived from Achromobacter, it is recombinantly expressed in Escherichia coli. This product is a serine protease that can highly specifically hydrolyze amides, esters, and peptide bonds on the carboxyl side of lysine. The optimal reaction pH is 9.0-9.5, and the optimal reaction temperature is 30-37°C. It can still maintain high enzyme activity in the presence of 4M urea or 0.1% SDS.
The storage solution is 20 mM Tris-HCl, pH 8.0.
Instructions
Protein digestion, the recommended digestion ratio is 1:50-1:200 (w/w, enzyme: protein), and digestion is carried out at 37°C overnight.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.4.21.50
Enzyme Activity
≥3.0 AU/mg soild
Unit Definition
One activity unit is defined as the amount of enzyme that catalyze the formation of 1 μmol AC-LyspNA in 1 min at 37℃ in Tris-HCl buffer at 25 mmol/L, pH 9.0
Technical Parameters
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Source
E.coli
Chemical Information
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CAS No. 123175-82-6
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Lysyl endopeptidase, Achromobacter sp]
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Synonyms
Lys-C
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
순도&문서
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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