Malpinin A
Malpinin A (MBJ-0173) is a dehydrohexapeptide biosurfactant with significant anthelmintic activity. Malpinin A specifically accumulates in the nematode digestive tract, thereby effectively inhibiting nematode feeding and proliferation without causing paralysis. Malpinin A can be used in studies related to plant-parasitic nematode infection.
Para uso exclusivo en investigación. No vendemos a pacientes.
- No. CAS: 2097822-76-7
- Fòrmula: C44H62N10O8
- Peso molecular:859.03
-
Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Malpinin A (100 μM) shows no significant cytotoxicity against SKOV-3, ACC-MESO-1, or Jurkat cells at a concentration of 100 μM[2].
Malpinin A (10 μg) shows no antibacterial activity against Micrococcus luteus at a dose of 10 μg per disk[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Malpinin A (100 μg mL-1; supplementation of fungal culture media) at 100 μg mL-1 confers nematode protection to M. indohii, significantly reducing Aphelenchus avenae propagation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:unspecified strain (adult hermaphrodites, stage L3−4 larvae)[1]
-
Dosage:5.45 μg mL-1 (IC50 concentration); 11.6 μM (2× IC50 concentration); 4× IC50 concentration
-
Administration:exposure in culture media
-
Result:Exhibited anthelminthic activity with an IC50 of 5.45 μg mL-1.
Killed or nearly immobilized nematodes at 4× IC50, with only very few larvae hatching compared to untreated controls.
Ceased nematode feeding on bacterial food sources without immediately affecting motility.
-
Animal Model:unspecified strain[1]
-
Dosage:100 μg mL-1
-
Administration:supplementation of fungal culture media
-
Result:Reduced *Aphelenchus avenae* propagation drastically, with nematode numbers comparable to levels seen in cocultures with *M. alpina* (natural malpinin producer).
-
Animal Model:unspecified strain (adult hermaphrodites, larvae)[1]
-
Dosage:16.92 μM (malpinin A-FAM, IC50); 110 μM (malpinin A-alkyne, Raman imaging); 31 μM (malpinin A-alkyne, full anthelminthic inhibition)
-
Administration:exposure in culture media
-
Result:Showed high fluorescence emission originating from the entire digestive tract, including the pharynx and grinder region, in both larvae and adults via malpinin A-FAM imaging.
Distinguished malpinin A-FAM signal from autofluorescence via FLIM, confirming accumulation in the intestinal lumen.
Confirmed accumulation in the pharyngeal lumen, parts of the grinder, and the intestine via alkyne-tagged malpinin A Raman microspectroscopy.
Chemical Information
-
No. CAS 2097822-76-7
-
Peso molecular 859.03
-
Fòrmula C44H62N10O8
-
Synonyms
MBJ-0173
-
Sequence
Ac-d{Leu-Arg-Leu}-Phe-{DHB}-d{Trp}
-
Sequence Shortening
Ac-d{LRL}-F-{DHB}-d{Trp}
-
Structure Classification
-
Initial Source
Mortierella alpina
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
-
Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Malpinin A Related Classifications
HY-P11862 Related Classifications
Powered by Bioz