Methylated porcine trypsin
Methylated porcine trypsin is a serine protease, which is divided into two categories: heterologously methylated type and homogeneously recombinantly methylated type. Methylated porcine trypsin hydrolyzes the C-terminal peptide bonds of lysine and arginine, and can block autolytic sites via reductive methylation modification of lysine residues, thereby inhibiting autoproteolysis. Methylated porcine trypsin is applicable to protein-related research.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Methylated porcine trypsin (Trypsin E) exhibits a 90% increase in the energy required for unfolding in the presence of 100 mM CaCl2, and retains thermal stability at temperatures up to 57 °C[1].
Methylated porcine trypsin exhibits superior autolysis resistance, with the intact enzyme peak area retention rate remaining >90% after incubation at 37 °C for 1 h, and the increment of autolytic peptides is extremely low[1].
Methylated porcine trypsin enables efficient peptide mapping analysis of infliximab; at an enzyme-to-protein ratio of 1:5 and after incubation at 37 °C for 30 min, it yields 96.4% of matched tryptic peptides with minimal autolysis interference[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Technical Parameters
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Source
E.coli
Chemical Information
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SMILES
[Methylated porcine trypsin]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Methylated porcine trypsin Related Classifications
HY-E71728 Related Classifications
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